Nucleic Acids Research, Vol 25, Issue 20 4093-4097, Copyright © 1997 by Oxford University Press
BC Persson, G Jager and C Gustafsson
We have evidence that the open reading frame previously denoted spoU is
necessary for tRNA (Gm18) 2'-O-methyltransferase activity. The spoU gene is
located in the gmk-rpoZ-spoT-spoU-recG operon at 82 minutes on the
Escherichia coli chromosome. The deduced amino acid sequence of spoU shows
strong similarities to previously characterized 2'-O- methyltransferases.
Comparison of the nucleoside modification pattern of hydrolyzed tRNA, 16S
rRNA and 23S rRNA from wild-type and spoU null mutants showed that the
modified nucleoside 2'-O-methylguanosine (Gm), present in a subset of E.
coli tRNAs at residue 18, is completely absent in the spoU mutant,
suggesting that spoU encodes tRNA (Gm18) 2'- O-methyltransferase.
Nucleoside modification of 16S and 23S rRNA was unaffected in the spoU
mutant. Insertions in the downstream recG gene did not affect RNA
modification. Absence of Gm18 in tRNA does not influence growth rate under
the tested conditions and does not interfere with activity of the SupF
amber suppressor, a suppressor tRNA that normally has the Gm18
modification. We suggest that the spoU gene be renamed trmH (tRNA
methylation).
ARTICLES
The spoU gene of Escherichia coli, the fourth gene of the spoT operon, is essential for tRNA (Gm18) 2'-O-methyltransferase activity
Department of Microbiology, Umea University, S-901 87 Umea, Sweden. bpersson@genetics.utah.edu
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Tomikawa, T. Yokogawa, T. Kanai, and H. Hori N7-Methylguanine at position 46 (m7G46) in tRNA from Thermus thermophilus is required for cell viability at high temperatures through a tRNA modification network Nucleic Acids Res., November 24, 2009; (2009) gkp1059v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Toyooka, T. Awai, T. Kanai, T. Imanaka, and H. Hori Stabilization of tRNA (m1G37) methyltransferase [TrmD] from Aquifex aeolicus by an intersubunit disulfide bond formation Genes Cells, August 1, 2008; 13(8): 807 - 816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Takeda, T. Toyooka, Y. Ikeuchi, S.-i. Yokobori, K. Okadome, F. Takano, T. Oshima, T. Suzuki, Y. Endo, and H. Hori The substrate specificity of tRNA (m1G37) methyltransferase (TrmD) from Aquifex aeolicus Genes Cells, December 1, 2006; 11(12): 1353 - 1365. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. BUJNICKI, Y. OUDJAMA, M. ROOVERS, S. OWCZAREK, J. CAILLET, and L. DROOGMANS Identification of a bifunctional enzyme MnmC involved in the biosynthesis of a hypermodified uridine in the wobble position of tRNA RNA, August 1, 2004; 10(8): 1236 - 1242. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Basha, G. J. Lee, L. A. Breci, A. C. Hausrath, N. R. Buan, K. C. Giese, and E. Vierling The Identity of Proteins Associated with a Small Heat Shock Protein during Heat Stress in Vivo Indicates That These Chaperones Protect a Wide Range of Cellular Functions J. Biol. Chem., February 27, 2004; 279(9): 7566 - 7575. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hori, S. Kubota, K. Watanabe, J.-M. Kim, T. Ogasawara, T. Sawasaki, and Y. Endo Aquifex aeolicus tRNA (Gm18) Methyltransferase Has Unique Substrate Specificity: tRNA RECOGNITION MECHANISM OF THE ENZYME J. Biol. Chem., June 27, 2003; 278(27): 25081 - 25090. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. G. S. De Bie, M. Roovers, Y. Oudjama, R. Wattiez, C. Tricot, V. Stalon, L. Droogmans, and J. M. Bujnicki The yggH Gene of Escherichia coli Encodes a tRNA (m7G46) Methyltransferase J. Bacteriol., May 15, 2003; 185(10): 3238 - 3243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Kinghorn, C. P. O'Byrne, I. R. Booth, and I. Stansfield Physiological analysis of the role of truB in Escherichia coli: a role for tRNA modification in extreme temperature resistance Microbiology, November 1, 2002; 148(11): 3511 - 3520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Urbonavicius, J. M. B. Durand, and G. R. Bjork Three Modifications in the D and T Arms of tRNA Influence Translation in Escherichia coli and Expression of Virulence Genes in Shigella flexneri J. Bacteriol., October 1, 2002; 184(19): 5348 - 5357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Anantharaman, E. V. Koonin, and L. Aravind Comparative genomics and evolution of proteins involved in RNA metabolism Nucleic Acids Res., April 1, 2002; 30(7): 1427 - 1464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Lovgren and P. M. Wikstrom The rlmB Gene Is Essential for Formation of Gm2251 in 23S rRNA but Not for Ribosome Maturation in Escherichia coli J. Bacteriol., December 1, 2001; 183(23): 6957 - 6960. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hori, N. Yamazaki, T. Matsumoto, Y.-i. Watanabe, T. Ueda, K. Nishikawa, I. Kumagai, and K. Watanabe Substrate Recognition of tRNA (Guanosine-2'-)-methyltransferase from Thermus thermophilus HB27 J. Biol. Chem., October 2, 1998; 273(40): 25721 - 25727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. B. Berlyn Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map Microbiol. Mol. Biol. Rev., September 1, 1998; 62(3): 814 - 984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-F. Cheng, Y. Zuo, Z. Li, K. E. Rudd, and M. P. Deutscher The vacB Gene Required for Virulence in Shigella flexneri and Escherichia coli Encodes the Exoribonuclease RNase R J. Biol. Chem., June 5, 1998; 273(23): 14077 - 14080. [Abstract] [Full Text] [PDF]
|
|