Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (55K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Li, C.
Right arrow Articles by Evans, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, C.
Right arrow Articles by Evans, R. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 25, Issue 20 4165-4166, Copyright © 1997 by Oxford University Press

ARTICLES

Ligation independent cloning irrespective of restriction site compatibility

C Li and RM Evans
Gene Expression Laboratory, The Salk Institute for Biological Studies, Howard Hughes Medical Institute, 10010 North Torrey Pines Road, La Jolla, CA 92186, USA. cli@axpl.salk.edu

Here we report the use of exonuclease to expose complementary DNA between an insert and vector such that annealing becomes independent of restriction site compatibility. We demonstrate that unusual and, in some cases, previously impossible cloning strategies can be readily and efficiently achieved as long as the flanking sequences of the linear vectors are highly related. Furthermore, we show that the bacterial repair system resolves the residual mismatches, overhangs or gaps in a predictable fashion to generate excisable inserts. This approach facilitates cloning regardless of restriction site compatibility and overcomes an important limitation in current cloning techniques.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J. Wang, T. Li, X. Guo, and Z. Lu
Exonuclease III protection assay with FRET probe for detecting DNA-binding proteins
Nucleic Acids Res., February 1, 2005; 33(2): e23 - e23.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
B. Blumberg, W. Sabbagh Jr., H. Juguilon, J. Bolado Jr., C. M. van Meter, E. S. Ong, and R. M. Evans
SXR, a novel steroid and xenobioticsensing nuclear receptor
Genes & Dev., October 15, 1998; 12(20): 3195 - 3205.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.