Nucleic Acids Research, Vol 25, Issue 21 4230-4234, Copyright © 1997 by Oxford University Press
L Wu and F Winston
The Snf-Swi complex of the yeast Saccharomyces cerevisiae has been shown to
control gene expression by controlling chromatin structure. We have
analyzed the promoter of the SUC2 gene, a gene strongly controlled by
Snf-Swi, by a high resolution analysis of micrococcal nuclease digests.
This analysis suggests that there are at least four nucleosomes positioned
over the SUC2 TATA and UAS regions under conditions repressing SUC2
transcription. Under derepressing conditions this entire promoter region is
much more sensitive to MNase digestion. Analysis of an snf2 Delta mutant
demonstrates that even under derepressing conditions the SUC2 promoter is
resistant to MNase digestion. Thus, the Snf-Swi complex appears to control
chromatin structure over both the SUC2 TATA and UAS regions. The presence
of nucleosomes over both promoter regions may explain the strong
requirement of SUC2 for Snf-Swi function.
ARTICLES
Evidence that Snf-Swi controls chromatin structure over both the TATA and UAS regions of the SUC2 promoter in Saccharomyces cerevisiae
Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
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