Nucleic Acids Research, Vol 25, Issue 21 4257-4263, Copyright © 1997 by Oxford University Press
MS Reagan and EC Friedberg
We have measured the kinetics of the recovery of mRNA synthesis in the
inducible GAL10 and RNR3 genes after exposure of yeast cells to ultraviolet
(UV) radiation. Such recovery is abolished in mutant strains defective in
nucleotide excision repair (NER) of DNA, including a rad23 mutant. Mutants
defective in the RAD7 or RAD16 genes, which are required for the repair of
the non-transcribed strand but not the transcribed strand of
transcriptionally active genes, show slightly faster recovery of RNA
synthesis than wild-type strains. A strain deleted of the RAD26 gene, which
is known to be required for strand- specific NER in yeast, manifested
delayed recovery of mRNA synthesis, whereas a rad28 mutant, which does not
show defective strand-specific repair, showed normal kinetics of recovery.
Measurement of the recovery of expression of selected individual yeast
genes by Northern analysis following exposure of cells to UV radiation
apparently correlates directly with the capacity of cells for
strand-specific NER.
ARTICLES
Recovery of RNA polymerase II synthesis following DNA damage in mutants of Saccharomyces cerevisiae defective in nucleotide excision repair
Laboratory of Molecular Pathology, Department of Pathology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75235, USA.
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