Nucleic Acids Research, Vol 25, Issue 21 4331-4337, Copyright © 1997 by Oxford University Press
NF Lue and Y Peng
A telomerase-like primer extension activity has been detected in
chromatographic fractions derived from Schizosaccharomyces pombe extracts.
This primer extension activity acts preferentially on dG-rich
oligodeoxynucleotides, is sensitive to RNase A pretreatment and requires
all four deoxynucleotides for optimal polymerization. The extension
products are also truncated by the inclusion of any one of the four
dideoxynucleotides, consistent with the presence of all four bases in the
S.pombe telomeric repeats. The intensity distribution of the extension
products and the dideoxynucleotide termination pattern suggest that
nucleotide addition is template directed, and that telomere-like sequences
are added to the primers. In particular, the sequence d(CGGTTA), a variant
of the S.pombe telomeric repeat, can be added directly by the in vitro
activity. Partially purified S.pombe telomerase sediments as a 35S
particle, suggesting that it exists in vivo as part of a large
multi-protein complex.
ARTICLES
Identification and characterization of a telomerase activity from Schizosaccharomyces pombe
Department of Microbiology, W. R. Hearst Microbiology Research Center, Cornell University Medical College, New York, NY 10021, USA.
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