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Nucleic Acids Research, Vol 25, Issue 22 4447-4454, Copyright © 1997 by Oxford University Press

ARTICLES

Oligonucleotide dendrimers: synthesis and use as polylabelled DNA probes

MS Shchepinov, IA Udalova, AJ Bridgman and EM Southern
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK. misha@bioch.ox.ac.uk

Oligonucleotide dendrimers were synthesized using a novel phosphoramidite synthon, tris-2,2,2-[3-(4,4'-dimethoxytrityloxy) propyloxymethyl]ethyl- N , N -diisopropylaminocyanethoxy phosphoramidite. Label, incorporated using [gamma-32P]ATP and polynucleotide kinase, was increased in proportion to the number of 5'- ends. There was a similar increase in signal when these multiply labelled oligonucleotides were used as probes to oligonucleotide arrays. A dendrimeric oligonucleotide was used successfully as a primer in the PCR. The strand bearing the dendrimer was resistant to degradation by T7 Gene 6 exonuclease making it easy to convert the double-stranded product of the PCR to a multiply-labelled, single- stranded probe.
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