Nucleic Acids Research, Vol 25, Issue 22 4513-4522, Copyright © 1997 by Oxford University Press
A Sureau, J Soret, C Guyon, C Gaillard, S Dumon, M Keller, P Crisanti and B Perbal
The PR264/SC35 splicing factor belongs to the family of SR proteins which
function as essential and alternative splicing factors. Here, we report
that the human PR264/SC35 locus is bidirectionally transcribed. Double in
situ hybridization experiments have allowed simultaneous detection of sense
and antisense RNA in human CCRF-CEM cells, suggesting that expression of
the corresponding genes is not mutually exclusive. We have characterized
three main classes of ET RNAs encoded by the opposite strand of the
PR264/SC35 gene and containing PR264/SC35- overlapping sequences,
PR264/SC35-non overlapping sequences or a combination of both. We show that
their expression results from the use of alternative promoters, exons and
polyadenylation signals. PR264/SC35- non overlapping ET mRNA species
potentially encode two protein isoforms (449 and 397 amino acids) and are
expressed from the PR264/SC35 promoting region. Northern blots and RNase
protection analyses indicate that ET polyadenylated RNAs are differentially
expressed in several human cell lines. Similar studies performed in the
mouse have revealed that the bidirectional transcription of the PR264/SC35
locus is a conserved mechanism and that the open reading frame identified
in a subset of human ET mRNAs is highly conserved (93% homology). Northern
blot analyses performed with several murine tissues confirmed the
differential expression of the ET gene and revealed that it is
predominantly expressed in the testis.
ARTICLES
Characterization of multiple alternative RNAs resulting from antisense transcription of the PR264/SC35 splicing factor gene
Laboratoire d'Oncologie Virale et Moleculaire, Unite de Formation et de Recherche de Biochimie, Universite Paris 7 (D. Diderot), 2 Place Jussieu, 75005 Paris, France.
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