Nucleic Acids Research, Vol 25, Issue 22 4599-4607, Copyright © 1997 by Oxford University Press
DA Adamiak, J Milecki, M Popenda, RW Adamiak, Z Dauter and WR Rypniewski
The molecular and crystal structure of 2'-O-Me (CGCGCG)2 has been
determined using synchrotron radiation at near-atomic resolution (1.30 A),
the highest resolution to date in the RNA field. The crystal structure is a
half-turn A-type helix with some helical parameters deviating from
canonical A-RNA, such as low base pair rise, elevated helical twist and
inclination angles. In CG steps, inter-strand guanines are parallel while
cytosines are not parallel. In steps GC this motif is reversed. This type
of regularity is not seen in other RNA crystal structures. The structure
includes 44 water molecules and two hydrated Mg2+ions one of which lies
exactly on the crystallographic 2-fold axis. There are distinct patterns of
hydration in the major and the minor grooves. The major groove is
stabilised by water clusters consisting of fused five- and six-membered
rings. Minor groove contains only a single row of water molecules; each
water bridges either two self-parallel cytosines or two self-parallel
guanines by a pair of hydrogen bonds. The structure provides the first view
of the hydration scheme of 2'-O-methylated RNA duplex.
ARTICLES
Crystal structure of 2'-O-Me(CGCGCG)2, an RNA duplex at 1.30 A resolution. Hydration pattern of 2'-O-methylated RNA
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland. dorota@tryptophan.ibch.poznan.pl
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