Nucleic Acids Research, Vol 25, Issue 23 4778-4785, Copyright © 1997 by Oxford University Press
D Ursic, KL Himmel, KA Gurley, F Webb and MR Culbertson
A single base change in the helicase superfamily 1 domain of the yeast
Saccharomyces cerevisiae SEN1 gene results in a heat-sensitive mutation
that alters the cellular abundance of many RNA species. We compared the
relative amounts of RNAs between cells that are wild-type and mutant after
temperature-shift. In the mutant several RNAs were found to either decrease
or increase in abundance. The affected RNAs include tRNAs, rRNAs and small
nuclear and nucleolar RNAs. Many of the affected RNAs have been positively
identified and include end-matured precursor tRNAs and the small nuclear
and nucleolar RNAs U5 and snR40 and snR45. Several small nucleolar RNAs
co-immunoprecipitate with Sen1 but differentially associate with the
wild-type and mutant protein. Its inactivation also impairs precursor rRNA
maturation, resulting in increased accumulation of 35S and 6S precursor
rRNAs and reduced levels of 20S, 23S and 27S rRNA processing intermediates.
Thus, Sen1 is required for the biosynthesis of various functionally
distinct classes of nuclear RNAs. We propose that Sen1 is an RNA helicase
acting on a wide range of RNA classes. Its effects on the targeted RNAs in
turn enable ribonuclease activity.
ARTICLES
The yeast SEN1 gene is required for the processing of diverse RNA classes
Laboratories of Molecular Biology and Genetics, 1525 Linden Drive, University of Wisconsin, Madison, WI 53706, USA. dursic@facstaff.wisc.edu
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