Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (304K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Yoshinaga, K.
Right arrow Articles by Ueno, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoshinaga, K.
Right arrow Articles by Ueno, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 25, Issue 23 4830-4834, Copyright © 1997 by Oxford University Press

ARTICLES

Extensive RNA editing and possible double-stranded structures determining editing sites in the atpB transcripts of hornwort chloroplasts

K Yoshinaga, T Kakehi, Y Shima, H Iinuma, T Masuzawa and M Ueno
Faculty of Science, Shizuoka University, Shizuoka 422, Japan. sckyosi@sci.shizuoka.ac.jp

Three nonsense codons and an unusual initiation codon were located within the putative coding region of the atpB gene of chloroplast DNA of the hornwort Anthoceros formosae. Nucleotide sequencing of cDNA prepared from transcripts revealed extensive RNA editing. The unusual initiation codon ACG was changed to AUG and three nonsense codons were converted into sense codons. In total 15 C residues of the genomic DNA were replaced by U residues in the mRNA sequences, while 14 U residues were replaced by C residues. This is the highest number of editing events for a chloroplast mRNA reported so far. Partial editing was also shown in a cDNA clone where 23 sites were edited but six sites remained unedited, representing the existence of premature mRNA. The expected two-dimensional structure of the mRNA shows the existence of a sequence complementary to every editing site, which can produce continuous base pairing longer than 5 bp, suggesting that mispairing in the double strand is the site determinant for RNA editing in Anthoceros chloroplasts. Comparison of the cDNA sequence with other chloroplast genes suggests that the mechanism arose in the first land plants and has been reduced during evolution.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
M. Kugita, Y. Yamamoto, T. Fujikawa, T. Matsumoto, and K. Yoshinaga
RNA editing in hornwort chloroplasts makes more than half the genes functional
Nucleic Acids Res., May 1, 2003; 31(9): 2417 - 2423.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Kugita, A. Kaneko, Y. Yamamoto, Y. Takeya, T. Matsumoto, and K. Yoshinaga
The complete nucleotide sequence of the hornwort (Anthoceros formosae) chloroplast genome: insight into the earliest land plants
Nucleic Acids Res., January 15, 2003; 31(2): 716 - 721.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
T. Miyamoto, J. Obokata, and M. Sugiura
Recognition of RNA Editing Sites Is Directed by Unique Proteins in Chloroplasts: Biochemical Identification of cis-Acting Elements and trans-Acting Factors Involved in RNA Editing in Tobacco and Pea Chloroplasts
Mol. Cell. Biol., October 1, 2002; 22(19): 6726 - 6734.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.