Nucleic Acids Research, Vol 25, Issue 23 4855-4857, Copyright © 1997 by Oxford University Press
GM Lamm, P Steinlein, M Cotten and G Christofori
We describe a rapid and quantitative flow cytometric method for determining
the apoptotic or anti-apoptotic potential of a gene in various cell types.
A plasmid carrying green fluorescent protein (GFP) is co-transfected with
an expression vector encoding the gene of interest. Subsequently cells are
stained with propidium iodide and, utilising flow cytometry, transfected,
GFP-expressing single cells are detected and apoptotic cells in this
population are identified by their DNA content of <2 N. The method
detects apoptosis as reliably as established methods using in situ nick-end
labelling but is faster, easier and less expensive.
ARTICLES
A rapid, quantitative and inexpensive method for detecting apoptosis by flow cytometry in transiently transfected cells
Research Institute of Molecular Pathology (I. M. P.), Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.
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