Nucleic Acids Research, Vol 25, Issue 24 4876-4882, Copyright © 1997 by Oxford University Press
JD Thompson, TJ Gibson, F Plewniak, F Jeanmougin and DG Higgins
CLUSTAL X is a new windows interface for the widely-used progressive
multiple sequence alignment program CLUSTAL W. The new system is easy to
use, providing an integrated system for performing multiple sequence and
profile alignments and analysing the results. CLUSTAL X displays the
sequence alignment in a window on the screen. A versatile sequence
colouring scheme allows the user to highlight conserved features in the
alignment. Pull-down menus provide all the options required for traditional
multiple sequence and profile alignment. New features include: the ability
to cut-and-paste sequences to change the order of the alignment, selection
of a subset of the sequences to be realigned, and selection of a sub-range
of the alignment to be realigned and inserted back into the original
alignment. Alignment quality analysis can be performed and low-scoring
segments or exceptional residues can be highlighted. Quality analysis and
realignment of selected residue ranges provide the user with a powerful
tool to improve and refine difficult alignments and to trap errors in input
sequences. CLUSTAL X has been compiled on SUN Solaris, IRIX5.3 on Silicon
Graphics, Digital UNIX on DECstations, Microsoft Windows (32 bit) for PCs,
Linux ELF for x86 PCs, and Macintosh PowerMac.
ARTICLES
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools
Institut de Genetique et de Biologie Moleculaire et Cellulaire (CNRS/INSERM/ULP), BP 163, 67404 Illkirch Cedex, France.
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