Nucleic Acids Research, Vol 25, Issue 24 4926-4932, Copyright © 1997 by Oxford University Press
J Zwicker, FC Lucibello, V Jerome, S Brusselbach and R Muller
The cdc25C , cyclin A and cdc2 genes are regulated during the cell cycle
through two contiguous repressor binding sites, the CDE and CHR, located in
the region of transcription initiation and interacting with a factor termed
CDF-1. The target of this repression seems to be transcriptional activation
of these promoters by transcription factors bound upstream. The majority of
these factors falls into the class of glutamine-rich activators, suggesting
that CDF-1-mediated repression might be activation domain specific. In the
present study we have used chimeric promoter constructs to demonstrate that
the cdc25C UAS, but not the core promoter, is crucial for repression. In
addition, we show that only specific transcription factors and activation
domains are responsive to CDE-CHR-mediated cell cycle regulation. These
observations clearly indicate that CDF-1 interferes with activation of
transcription by a specific subset of transactivators. The repressible
activation domains belong to the same class of glutamine-rich activators,
pointing to specific interactions of CDF-1 with components of the
transcription machinery. In agreement with this conclusion we find that a
simple inversion of the CDE-CHR module completely abrogates cell
cycle-regulated repression.
ARTICLES
CDF-1-mediated repression of cell cycle genes targets a specific subset of transactivators [corrected and republished in Nucleic Acids Res 1998 Feb 15;26(4):4926-32]
Institut fur Molekularbiologie und Tumorforschung (IMT), Philipps- Universitat Marburg, Emil-Mannkopff-Strasse 2, D-35033 Marburg, Germany.
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