Nucleic Acids Research, Vol 25, Issue 24 5003-5009, Copyright © 1997 by Oxford University Press
DT Brown, A Gunjan, BT Alexander and DB Sittman
The in vivo overproduction of two mouse histone H1 variants in homologous
mouse fibroblasts has opposite effects on gene expression. Overproduction
of H1(0) results in repression of transcript levels of all polymerase II
genes tested. In contrast, overproduction of H1c results in elevated levels
of transcripts. We created a series of chimeric H1 genes in which the
regions encoding the three structural domains common to this family of
these proteins were systematically switched. Overexpression of these genes
in vivo resulted in the accumulation of large amounts of the chimeric H1 in
chromatin. Analysis of the effects of overproduction of these proteins
revealed that the differential effect of H1 variant overproduction on gene
expression is due to differences in the central globular domain.
ARTICLES
Differential effect of H1 variant overproduction on gene expression is due to differences in the central globular domain
Department of Biochemistry, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216-4505, USA. dtbrown@fiona.umsmed.edu
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Chen, M. Dundr, C. Wang, A. Leung, A. Lamond, T. Misteli, and S. Huang Condensed mitotic chromatin is accessible to transcription factors and chromatin structural proteins J. Cell Biol., January 3, 2005; 168(1): 41 - 54. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Cheung, A. S. Zarifyan, and W. L. Kraus Histone H1 Represses Estrogen Receptor {alpha} Transcriptional Activity by Selectively Inhibiting Receptor-Mediated Transcription Initiation Mol. Cell. Biol., April 15, 2002; 22(8): 2463 - 2471. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. ZLATANOVA, P. CAIAFA, and K. VAN HOLDE Linker histone binding and displacement: versatile mechanism for transcriptional regulation FASEB J, September 1, 2000; 14(12): 1697 - 1704. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. Adenot, E Campion, E Legouy, C. Allis, S Dimitrov, J Renard, and E. Thompson Somatic linker histone H1 is present throughout mouse embryogenesis and is not replaced by variant H1 degrees J. Cell Sci., January 8, 2000; 113(16): 2897 - 2907. [Abstract] [PDF]
|
|
|
|
 |
|
 A. Gunjan, B. T. Alexander, D. B. Sittman, and D. T. Brown Effects of H1 Histone Variant Overexpression on Chromatin Structure J. Biol. Chem., December 31, 1999; 274(53): 37950 - 37956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Carruthers and J. C. Hansen The Core Histone N Termini Function Independently of Linker Histones during Chromatin Condensation J. Biol. Chem., November 17, 2000; 275(47): 37285 - 37290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gunjan, D. B. Sittman, and D. T. Brown Core Histone Acetylation Is Regulated by Linker Histone Stoichiometry in Vivo J. Biol. Chem., January 26, 2001; 276(5): 3635 - 3640. [Abstract] [Full Text] [PDF]
|
|