Nucleic Acids Research, Vol 25, Issue 3 518-522, Copyright © 1997 by Oxford University Press
L Barbieri, P Valbonesi, E Bonora, P Gorini, A Bolognesi and F Stirpe
Ribosome-inactivating proteins (RIP) are a family of plant enzymes for
which a unique activity was determined: rRNAN-glycosidase at a specific
universally conserved position, A4324in the case of rat ribosomes. Recently
we have shown that the RIP from Saponaria officinalis have a much wider
substrate specificity: they are actually polynucleotide:adenosine
glycosidases. Here we extend studies on substrate specificity to most known
RIP: 52 purified proteins, both type 1 (single-chain) and type 2 (two
chain, an enzymatic chain and a lectin chain) were examined for adenine
release on various substrates including RNAs from different sources, DNA,
and poly(A). All RIP depurinated extensively DNA and some released adenine
from all adenine- containing polynucleotides tested. From experimental
evidence the entire class of plant proteins, up to now called
ribosome-inactivating proteins, may be classified as
polynucleotide:adenosine glycosidases. The newly identified substrates may
be implicated in the biological role(s) of RIP.
ARTICLES
Polynucleotide:adenosine glycosidase activity of ribosome-inactivating proteins: effect on DNA, RNA and poly(A)
Dipartimento di Patologia sperimentale dell'Universita degli Studi di Bologna Via San Giacomo 14, I-40126 Bologna, Italy. barbieri@alma.unibo.it
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M.-C. Ho, M. B. Sturm, S. C. Almo, and V. L. Schramm Transition state analogues in structures of ricin and saporin ribosome-inactivating proteins PNAS, December 1, 2009; 106(48): 20276 - 20281. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Parikh, A. Tortora, X.-P. Li, and N. E. Tumer Ricin Inhibits Activation of the Unfolded Protein Response by Preventing Splicing of the HAC1 mRNA J. Biol. Chem., March 7, 2008; 283(10): 6145 - 6153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Committee on Environmental Health and Committee on Chemical-Biological Terrorism and Its Impact on Children Pediatrics, September 1, 2006; 118(3): 1267 - 1278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Mishra, S. Bilgrami, R. S. Sharma, P. Kaur, S. Yadav, R. Krauspenhaar, C. Betzel, W. Voelter, C. R. Babu, and T. P. Singh Crystal Structure of Himalayan Mistletoe Ribosome-inactivating Protein Reveals the Presence of a Natural Inhibitor and a New Functionally Active Sugar-binding Site J. Biol. Chem., May 27, 2005; 280(21): 20712 - 20721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-W. Park, R. Vepachedu, R. A. Owens, and J. M. Vivanco The N-Glycosidase Activity of the Ribosome-inactivating Protein ME1 Targets Single-stranded Regions of Nucleic Acids Independent of Sequence or Structural Motifs J. Biol. Chem., August 13, 2004; 279(33): 34165 - 34174. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sharma, S.-W. Park, R. Vepachedu, L. Barbieri, M. Ciani, F. Stirpe, B. J. Savary, and J. M. Vivanco Isolation and Characterization of an RIP (Ribosome-Inactivating Protein)-Like Protein from Tobacco with Dual Enzymatic Activity Plant Physiology, January 1, 2004; 134(1): 171 - 181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bagga, D. Seth, and J. K. Batra The Cytotoxic Activity of Ribosome-inactivating Protein Saporin-6 Is Attributed to Its rRNA N-Glycosidase and Internucleosomal DNA Fragmentation Activities J. Biol. Chem., February 7, 2003; 278(7): 4813 - 4820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Parikh, C. Coetzer, and N. E. Tumer Pokeweed Antiviral Protein Regulates the Stability of Its Own mRNA by a Mechanism That Requires Depurination but Can Be Separated from Depurination of the alpha -Sarcin/Ricin Loop of rRNA J. Biol. Chem., October 25, 2002; 277(44): 41428 - 41437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bolognesi, L. Polito, C. Lubelli, L. Barbieri, A. Parente, and F. Stirpe Ribosome-inactivating and Adenine Polynucleotide Glycosylase Activities in Mirabilis jalapa L. Tissues J. Biol. Chem., April 12, 2002; 277(16): 13709 - 13716. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. BRIGOTTI, R. ALFIERI, P. SESTILI, M. BONELLI, P. G. PETRONINI, A. GUIDARELLI, L. BARBIERI, F. STIRPE, and S. SPERTI Damage to nuclear DNA induced by Shiga toxin 1 and ricin in human endothelial cells FASEB J, March 1, 2002; 16(3): 365 - 372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. J. PEUMANS, Q. HAO, and E. J. M. VAN DAMME Ribosome-inactivating proteins from plants: more than RNA N-glycosidases? FASEB J, July 1, 2001; 15(9): 1493 - 1506. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Brigotti, D. Carnicelli, P. Accorsi, S. Rizzi, L. Montanaro, and S. Sperti 4-Aminopyrazolo[3,4-d]pyrimidine (4-APP) as a novel inhibitor of the RNA and DNA depurination induced by Shiga toxin 1 Nucleic Acids Res., June 15, 2000; 28(12): 2383 - 2388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Delius and G. Adams Shock Wave Permeabilization with Ribosome Inactivating Proteins: A New Approach to Tumor Therapy Cancer Res., October 1, 1999; 59(20): 5227 - 5232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Nicolas, J. M. Beggs, B. M. Haltiwanger, and T. F. Taraschi A New Class of DNA Glycosylase/Apurinic/Apyrimidinic Lyases That Act on Specific Adenines in Single-stranded DNA J. Biol. Chem., July 3, 1998; 273(27): 17216 - 17220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Nicolas, J. M. Beggs, and T. F. Taraschi Gelonin Is an Unusual DNA Glycosylase That Removes Adenine from Single-stranded DNA, Normal Base Pairs and Mismatches J. Biol. Chem., September 29, 2000; 275(40): 31399 - 31406. [Abstract] [Full Text] [PDF]
|
|