Nucleic Acids Research, Vol 25, Issue 3 530-537, Copyright © 1997 by Oxford University Press
Y Okuno, T Okazaki and H Masukata
We have identified five autonomously replicating sequences (ARSs) in a 100
kbp region of the Schizosaccharomyces pombe chromosome II. Analyses of
replicative intermediates of the chromosome DNA by neutral/neutral
two-dimensional gel electrophoresis demonstrated that at least three of
these ARS loci operate as chromosomal replication origins. One of the
loci,ori2004, was utilized in almost every cell cycle, while the others
were used less frequently. The frequency of initiation from the respective
chromosomal replication origin was found to be roughly proportional to the
efficiency of autonomous replication of the corresponding ARS plasmid.
Replication from ori2004 was initiated within a distinct region almost the
same as that for replication of the ARS plasmid. These results showed that
the ori2004 region of approximately 3 kbp contains all the cis elements
essential for initiation of chromosome replication.
ARTICLES
Identification of a predominant replication origin in fission yeast
Department of Molecular Biology, School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 564-01, Japan.
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