Nucleic Acids Research, Vol 25, Issue 3 530-537, Copyright © 1997 by Oxford University Press
Y Okuno, T Okazaki and H Masukata
We have identified five autonomously replicating sequences (ARSs) in a 100
kbp region of the Schizosaccharomyces pombe chromosome II. Analyses of
replicative intermediates of the chromosome DNA by neutral/neutral
two-dimensional gel electrophoresis demonstrated that at least three of
these ARS loci operate as chromosomal replication origins. One of the
loci,ori2004, was utilized in almost every cell cycle, while the others
were used less frequently. The frequency of initiation from the respective
chromosomal replication origin was found to be roughly proportional to the
efficiency of autonomous replication of the corresponding ARS plasmid.
Replication from ori2004 was initiated within a distinct region almost the
same as that for replication of the ARS plasmid. These results showed that
the ori2004 region of approximately 3 kbp contains all the cis elements
essential for initiation of chromosome replication.
ARTICLES
Identification of a predominant replication origin in fission yeast
Department of Molecular Biology, School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 564-01, Japan.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Nitani, C. Yadani, H. Yabuuchi, H. Masukata, and T. Nakagawa Mcm4 C-terminal domain of MCM helicase prevents excessive formation of single-stranded DNA at stalled replication forks PNAS, September 2, 2008; 105(35): 12973 - 12978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Marilley, P. Milani, J. Thimonier, J. Rocca-Serra, and G. Baldacci Atomic force microscopy of DNA in solution and DNA modelling show that structural properties specify the eukaryotic replication initiation site Nucleic Acids Res., November 29, 2007; 35(20): 6832 - 6845. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Nitani, K.-i. Nakamura, C. Nakagawa, H. Masukata, and T. Nakagawa Regulation of DNA Replication Machinery by Mrc1 in Fission Yeast Genetics, September 1, 2006; 174(1): 155 - 165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Dai, R.-Y. Chuang, and T. J. Kelly DNA replication origins in the Schizosaccharomyces pombe genome PNAS, January 11, 2005; 102(2): 337 - 342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yamada, T. Nakagawa, and H. Masukata A Novel Intermediate in Initiation Complex Assembly for Fission Yeast DNA Replication Mol. Biol. Cell, August 1, 2004; 15(8): 3740 - 3750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Nakajima and H. Masukata SpSld3 Is Required for Loading and Maintenance of SpCdc45 on Chromatin in DNA Replication in Fission Yeast Mol. Biol. Cell, May 1, 2002; 13(5): 1462 - 1472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Irelan, G. I. Gutkin, and L. Clarke Functional Redundancies, Distinct Localizations and Interactions Among Three Fission Yeast Homologs of Centromere Protein-B Genetics, March 1, 2001; 157(3): 1191 - 1203. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. A. Bryant, K. Moore, and S. J. Aves Origins and complexes: the initiation of DNA replication J. Exp. Bot., February 1, 2001; 52(355): 193 - 202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Tsutsui, T. Morishita, H. Iwasaki, H. Toh, and H. Shinagawa A Recombination Repair Gene of Schizosaccharomyces pombe, rhp57, Is a Functional Homolog of the Saccharomyces cerevisiae RAD57 Gene and Is Phylogenetically Related to the Human XRCC3 Gene Genetics, April 1, 2000; 154(4): 1451 - 1461. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. Okuno, H. Satoh, M. Sekiguchi, and H. Masukata Clustered Adenine/Thymine Stretches Are Essential for Function of a Fission Yeast Replication Origin Mol. Cell. Biol., October 1, 1999; 19(10): 6699 - 6709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ogawa, T. Takahashi, and H. Masukata Association of Fission Yeast Orp1 and Mcm6 Proteins with Chromosomal Replication Origins Mol. Cell. Biol., October 1, 1999; 19(10): 7228 - 7236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Uchiyama, D. Griffiths, K.-i. Arai, and H. Masai Essential Role of Sna41/Cdc45 in Loading of DNA Polymerase alpha onto Minichromosome Maintenance Proteins in Fission Yeast J. Biol. Chem., July 6, 2001; 276(28): 26189 - 26196. [Abstract] [Full Text] [PDF]
|
|