Nucleic Acids Research, Vol 25, Issue 4 694-700, Copyright © 1997 by Oxford University Press
MF Dubois, M Vincent, M Vigneron, J Adamczewski, JM Egly and O Bensaude
The C-terminal domain (CTD) of the RNA polymerase II largest subunit (RPB1)
plays a central role in transcription. The CTD is unphosphorylated when the
polymerase assembles into a preinitiation complex of transcription and
becomes heavily phosphorylated during promoter clearance and entry into
elongation of transcription. A kinase associated to the general
transcription factor TFIIH, in the preinitiation complex, phosphorylates
the CTD. The TFIIH-associated CTD kinase activity was found to decrease in
extracts from heat-shocked HeLa cells compared to unstressed cells. This
loss of activity correlated with a decreased solubility of the TFIIH
factor. The TFIIH- kinase impairment during heat-shock was accompanied by
the disappearance of a particular phosphoepitope (CC-3) on the RPB1
subunit. The CC-3 epitope was localized on the C-terminal end of the CTD
and generated in vitro when the RPB1 subunit was phosphorylated by the
TFIIH-associated kinase but not by another CTD kinase such as MAP kinase.
In apparent discrepancy, the overall RPB1 subunit phosphorylation increased
during heat-shock. The decreased activity in vivo of the TFIIH kinase might
be compensated by a stress-activated CTD kinase such as MAP kinase. These
results also suggest that heat-shock gene transcription may have a weak
requirement for TFIIH kinase activity.
ARTICLES
Heat-shock inactivation of the TFIIH-associated kinase and change in the phosphorylation sites on the C-terminal domain of RNA polymerase II
Laboratoire de Genetique Moleculaire, URA CNRS 1302, Ecole Normale Superieure, Paris, France.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Meinhart, T. Kamenski, S. Hoeppner, S. Baumli, and P. Cramer A structural perspective of CTD function Genes & Dev., June 15, 2005; 19(12): 1401 - 1415. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Millhouse and J. L. Manley The C-Terminal Domain of RNA Polymerase II Functions as a Phosphorylation-Dependent Splicing Activator in a Heterologous Protein Mol. Cell. Biol., January 15, 2005; 25(2): 533 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. J. Iborra, A. E. Escargueil, K. Y. Kwek, A. Akoulitchev, and P. R. Cook Molecular cross-talk between the transcription, translation, and nonsense-mediated decay machineries J. Cell Sci., February 22, 2004; 117(6): 899 - 906. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. S. Lin, M.-F. Dubois, and M. E. Dahmus TFIIF-associating Carboxyl-terminal Domain Phosphatase Dephosphorylates Phosphoserines 2 and 5 of RNA Polymerase II J. Biol. Chem., November 22, 2002; 277(48): 45949 - 45956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Korsisaari, D. J. Rossi, A. Paetau, P. Charnay, M. Henkemeyer, and T. P. Makela Conditional ablation of the Mat1 subunit of TFIIH in Schwann cells provides evidence that Mat1 is not required for general transcription J. Cell Sci., November 15, 2002; 115(22): 4275 - 4284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Prelich RNA Polymerase II Carboxy-Terminal Domain Kinases: Emerging Clues to Their Function Eukaryot. Cell, April 1, 2002; 1(2): 153 - 162. [Full Text] [PDF]
|
|
|
|
|
|
A. Mathew, S. K. Mathur, C. Jolly, S. G. Fox, S. Kim, and R. I. Morimoto Stress-Specific Activation and Repression of Heat Shock Factors 1 and 2 Mol. Cell. Biol., November 1, 2001; 21(21): 7163 - 7171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Palancade, S. Bellier, G. Almouzni, and O. Bensaude Incomplete RNA polymerase II phosphorylation in Xenopus laevis early embryos J. Cell Sci., January 7, 2001; 114(13): 2483 - 2489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Casse, F. Giannoni, V. T. Nguyen, M.-F. Dubois, and O. Bensaude The Transcriptional Inhibitors, Actinomycin D and alpha -Amanitin, Activate the HIV-1 Promoter and Favor Phosphorylation of the RNA Polymerase II C-terminal Domain J. Biol. Chem., June 4, 1999; 274(23): 16097 - 16106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A Albert, S Lavoie, and M Vincent A hyperphosphorylated form of RNA polymerase II is the major interphase antigen of the phosphoprotein antibody MPM-2 and interacts with the peptidyl-prolyl isomerase Pin1 J. Cell Sci., January 8, 1999; 112(15): 2493 - 2500. [Abstract] [PDF]
|
|
|
|
|
|
M. Patturajan, R. J. Schulte, B. M. Sefton, R. Berezney, M. Vincent, O. Bensaude, S. L. Warren, and J. L. Corden Growth-related Changes in Phosphorylation of Yeast RNA Polymerase II J. Biol. Chem., February 20, 1998; 273(8): 4689 - 4694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Sandaltzopoulos and P. B. Becker Heat Shock Factor Increases the Reinitiation Rate from Potentiated Chromatin Templates Mol. Cell. Biol., January 1, 1998; 18(1): 361 - 367. [Abstract] [Full Text]
|
|
|
|
|
|
B. Grondin, F. Cote, M. Bazinet, M. Vincent, and M. Aubry Direct Interaction of the KRAB/Cys2-His2 Zinc Finger Protein ZNF74 with a Hyperphosphorylated Form of the RNA Polymerase II Largest Subunit J. Biol. Chem., October 31, 1997; 272(44): 27877 - 27885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Reyes, C. Muchardt, and M. Yaniv Components of the Human SWI/SNF Complex Are Enriched in Active Chromatin and Are Associated with the Nuclear Matrix J. Cell Biol., April 21, 1997; 137(2): 263 - 274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Busso, A. Keriel, B. Sandrock, A. Poterszman, O. Gileadi, and J.-M. Egly Distinct Regions of MAT1 Regulate cdk7 Kinase and TFIIH Transcription Activities J. Biol. Chem., July 21, 2000; 275(30): 22815 - 22823. [Abstract] [Full Text] [PDF]
|
|