Nucleic Acids Research, Vol 25, Issue 4 781-786, Copyright © 1997 by Oxford University Press
BS Singer, T Shtatland, D Brown and L Gold
An increasing number of proteins are being identified that regulate gene
expression by binding specific nucleic acidsin vivo. A method termed
genomic SELEX facilitates the rapid identification of networks of
protein-nucleic acid interactions by identifying within the genomic
sequences of an organism the highest affinity sites for any protein of the
organism. As with its progenitor, SELEX of random-sequence nucleic acids,
genomic SELEX involves iterative binding, partitioning, and amplification
of nucleic acids. The two methods differ in that the variable region of the
nucleic acid library for genomic SELEX is derived from the genome of an
organism. We have used a quick and simple method to construct Escherichia
coli, Saccharomyces cerevisiae, and human genomic DNA PCR libraries that
can be transcribed with T7 RNA polymerase. We present evidence that the
libraries contain overlapping inserts starting at most of the positions
within the genome, making these libraries suitable for genomic SELEX.
ARTICLES
Libraries for genomic SELEX [published erratum appears in Nucleic Acids Res 1997 Nov 1;25(21):4430]
Department of Molecular Biology, University of Colorado, Boulder 80309- 0347, USA.
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