Nucleic Acids Research, Vol 25, Issue 4 843-849, Copyright © 1997 by Oxford University Press
JL Kalenik, D Chen, ME Bradley, SJ Chen and TC Lee
Muscle-restricted transcription of sarcomeric actin genes is negatively
controlled by the zinc finger protein YY1, which is down-regulated at the
protein level during myogenic differentiation. To identify cellular
proteins that might mediate the function/stability of YY1 in muscle cells,
we screened an adult human muscle cDNA library using the yeast two-hybrid
cloning system. We report the isolation and characterization of a novel
protein termed YAF2 (YY1- associated factor 2) that interacts with YY1. The
YAF2 cDNA encodes a 180 amino acid basic protein (pI 10.5) containing a
single N-terminal C2-X10-C2 zinc finger. Lysine clusters are present that
may function as a nuclear localization signal. Domain mapping analysis
shows that the first and second zinc fingers of YY1 are targeted for YAF2
protein interaction. In contrast to the down-regulation of YY1, YAF2
message levels increase during in vitro differentiation of both rat
skeletal and cardiac muscle cells. YAF2 appears to have a promyogenic
regulatory role, since overexpression of YAF2 in C2 myoblasts stimulates
myogenic promoter activity normally restricted by YY1. Co-transfection of
YY1 reverses the stimulatory effect of YAF2. YAF2 also greatly potentiates
proteolytic cleavage of YY1 by the calcium- activated protease m- calpain.
The isolation of YAF2 may help in understanding the mechanisms through
which inhibitors of myogenic transcription may be antagonized or eliminated
by proteolysis during muscle development.
ARTICLES
Yeast two-hybrid cloning of a novel zinc finger protein that interacts with the multifunctional transcription factor YY1
Department of Biochemistry, SUNY at Buffalo, NY 14214, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Lin, J. McGrath, P. Wang, and T. Lee Cellular Toxicity Induced by SRF-Mediated Transcriptional Squelching Toxicol. Sci., March 1, 2007; 96(1): 83 - 91. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Dekel, L. Zangi, E. Shezen, S. Reich-Zeliger, S. Eventov-Friedman, H. Katchman, J. Jacob-Hirsch, N. Amariglio, G. Rechavi, R. Margalit, et al. Isolation and Characterization of Nontubular Sca-1+Lin- Multipotent Stem/Progenitor Cells from Adult Mouse Kidney J. Am. Soc. Nephrol., December 1, 2006; 17(12): 3300 - 3314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Stanton, L. J. McReynolds, T. Evans, and N. Schreiber-Agus Yaf2 Inhibits Caspase 8-mediated Apoptosis and Regulates Cell Survival during Zebrafish Embryogenesis J. Biol. Chem., September 29, 2006; 281(39): 28782 - 28793. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Pirity, J. Locker, and N. Schreiber-Agus Rybp/DEDAF Is Required for Early Postimplantation and for Central Nervous System Development Mol. Cell. Biol., August 15, 2005; 25(16): 7193 - 7202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Perezgasga, J. Jiang, B. Bolival Jr, M. Hiller, E. Benson, M. T. Fuller, and H. White-Cooper Regulation of transcription of meiotic cell cycle and terminal differentiation genes by the testis-specific Zn-finger protein matotopetli Development, April 15, 2004; 131(8): 1691 - 1702. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Palko, H. W. Bass, M. J. Beyrouthy, and M. M. Hurt The Yin Yang-1 (YY1) protein undergoes a DNA-replication-associated switch in localization from the cytoplasm to the nucleus at the onset of S phase J. Cell Sci., January 22, 2004; 117(3): 465 - 476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sawa, T. Yoshikawa, F. Matsuda-Suzuki, S. Delehouzee, M. Goto, H. Watanabe, J.-i. Sawada, K. Kataoka, and H. Handa YEAF1/RYBP and YAF-2 Are Functionally Distinct Members of a Cofactor Family for the YY1 and E4TF1/hGABP Transcription Factors J. Biol. Chem., June 14, 2002; 277(25): 22484 - 22490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ogawa, K.-i. Ishiguro, S. Gaubatz, D. M. Livingston, and Y. Nakatani A Complex with Chromatin Modifiers That Occupies E2F- and Myc-Responsive Genes in G0 Cells Science, May 10, 2002; 296(5570): 1132 - 1136. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Raab, B. Bauer, A. Gigler, K. Beckenlehner, H. Wolf, and S. Modrow Cellular transcription factors that interact with p6 promoter elements of parvovirus B19 J. Gen. Virol., June 1, 2001; 82(6): 1473 - 1480. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. TONKO, M. J. AUSSERLECHNER, D. BERNHARD, A. HELMBERG, and R. KOFLER Gene expression profiles of proliferating vs. G1/G0 arrested human leukemia cells suggest a mechanism for glucocorticoid-induced apoptosis FASEB J, March 1, 2001; 15(3): 693 - 699. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.R. Hiltkel, T.-C. Lee, and L.A. Bobekl Structure/Function Analysis of Human Cystatin SN and Comparison of the Cysteine Proteinase Inhibitory Profiles of Human Cystatins C and SN Journal of Dental Research, August 1, 1999; 78(8): 1401 - 1409. [Abstract] [PDF]
|
|
|
|
|
|
F. Galvagni, E. Cartocci, and S. Oliviero The Dystrophin Promoter Is Negatively Regulated by YY1 in Undifferentiated Muscle Cells J. Biol. Chem., December 11, 1998; 273(50): 33708 - 33713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Bird, J. S. Turner-Cavet, J. H. Lakey, and N. J. Robinson A Carboxyl-terminal Cys2/His2-type Zinc-finger Motif in DNA Primase Influences DNA Content in Synechococcus PCC 7942 J. Biol. Chem., August 14, 1998; 273(33): 21246 - 21252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Vassias, U. Hazan, Y. Michel, C. Sawa, H. Handa, L. Gouya, and F. Morinet Regulation of Human B19 Parvovirus Promoter Expression by hGABP (E4TF1) Transcription Factor J. Biol. Chem., April 3, 1998; 273(14): 8287 - 8293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Walowitz, M. E. Bradley, S. Chen, and T. Lee Proteolytic Regulation of the Zinc Finger Transcription Factor YY1, a Repressor of Muscle-restricted Gene Expression J. Biol. Chem., March 20, 1998; 273(12): 6656 - 6661. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zheng, O. Schickling, M. E. Peter, and M. J. Lenardo The Death Effector Domain-associated Factor Plays Distinct Regulatory Roles in the Nucleus and Cytoplasm J. Biol. Chem., August 17, 2001; 276(34): 31945 - 31952. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Trimarchi, B. Fairchild, J. Wen, and J. A. Lees The E2F6 transcription factor is a component of the mammalian Bmi1-containing polycomb complex PNAS, February 13, 2001; 98(4): 1519 - 1524. [Abstract] [Full Text] [PDF]
|
|