Nucleic Acids Research, Vol 25, Issue 5 1015-1021, Copyright © 1997 by Oxford University Press
JT Reardon, LH Thompson and A Sancar
Mammalian nucleotide excision repair is the primary enzymatic pathway for
removing bulky lesions from DNA. The repair reaction involves three main
steps: (i) dual incisions on both sides of the lesion; (ii) excision of the
damaged base in an oligonucleotide 24-31 nt in length; (iii) filling in of
the post-excision gap and ligation. We have developed assays that probe the
individual steps of the reaction. Using these methods (assays for incision,
excision and repair patch synthesis), we demonstrate that the mammalian
excision nuclease system removes bulky lesions by incising mainly at the
22nd-25th phosphodiester bonds 5'and the 3rd-5th phosphodiester bonds 3'of
the lesion, thus releasing oligonucleotides primarily 26-29 nt in length.
The resulting excision gap is filled in by DNA polymerases delta and
epsilon as revealed by the 'phosphorothioate repair patch assay'. When
these assays were employed with cell-free extracts from the moderately
UV-sensitive rodent mutants in complementation groups 6-10, we found that
these mutants are essentially normal in all three steps of the repair
reaction. This leads us to conclude that these cell lines have normal in
vitro repair activities and that the defects in these mutants are most
likely in genes controlling cellular functions not directly involved in
general excision repair.
ARTICLES
Rodent UV-sensitive mutant cell lines in complementation groups 6-10 have normal general excision repair activity
Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599-7260, USA.
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