Nucleic Acids Research, Vol 25, Issue 5 1036-1041, Copyright © 1997 by Oxford University Press
L Vayssie, L Sperling and L Madeddu
In Paramecium, as in other ciliates, the transcriptionally active
macronucleus is derived from the germline micronucleus by programmed DNA
rearrangements, which include the precise excision of thousands of
germline-specific sequences (internal eliminated sequences, IESs). We
report the characterization of micronuclear versions of genes encoding
Paramecium secretory granule proteins (trichocyst matrix proteins, TMPs)
and Paramecium centrins. TMP and centrin multigene families, previously
studied in the macronuclear genome, consist of genes that are co-expressed
to provide mixtures of related polypeptides that co- assemble to form
respectively the crystalline trichocyst matrix and the infraciliary
lattice, a contractile cytoskeletal network. We present evidence that TMP
and centrin genes identified in the macronucleus are also present in the
micronucleus, ruling out the possibility that these novel multigene
families are generated by somatic rearrangements during macronuclear
development. No IESs were found in TMP genes, however, four IESs in or near
germline centrin genes were characterized. The only intragenic IES is 75 bp
in size, interrupts a 29 bp intron and is absent from at least one other
closely related centrin gene. This is the first report of an IES in an
intron in Paramecium.
ARTICLES
Characterization of multigene families in the micronuclear genome of Paramecium tetraurelia reveals a germline specific sequence in an intron of a centrin gene
Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette Cedex, France.
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