Nucleic Acids Research, Vol 25, Issue 5 1078-1079, Copyright © 1997 by Oxford University Press
A Hoffmann, M Villalba, L Journot and D Spengler
The tetracycline-dependent expression system has gained increasing
popularity for the expression of any gene of interest. Careful choice of
the expression vector has been suggested to exploit the full potential of
this system. A novel tetracycline-sensitive expression vector based on a
modified mouse mammary tumor virus promoter achieved considerably improved
regulatory properties in a series of cell lines tested under transient and
stable conditions. Therefore, the applicability of the
tetracycline-dependent expression system can be largely enhanced by careful
adaptation of the expression vector to the host cell line.
ARTICLES
A novel tetracycline-dependent expression vector with low basal expression and potent regulatory properties in various mammalian cell lines
Max Planck Institute of Psychiatry, Molecular Neurobiology, Kraepelinstrasse 2-10, D-80804 Munich, Germany.
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