Nucleic Acids Research, Vol 25, Issue 5 925-932, Copyright © 1997 by Oxford University Press
AM Borman, P Le Mercier, M Girard and KM Kean
We recently compared the efficiency of six picornaviral internal ribosome
entry segments (IRESes) and the hepatitis C virus (HCV) IRES for their
ability to drive internal initiation of translationin vitro. Here we
present the results of a similar comparison performed in six different
cultured cell lines infected with a recombinant vaccinia virus expressing
the T7 polymerase and transfected with dicistronic plasmids. The IRESes
could be divided into three groups: (i) the cardiovirus and aphthovirus
IRESes (and the HCV element) direct internal initiation efficiently in all
cell lines tested; (ii) the enterovirus and rhinovirus IRESes are at least
equally efficient in several cell lines, but are extremely inefficient in
certain cell types; and (iii) the hepatitis A virus IRES is incapable of
directing efficient internal initiation in any of the cell lines used
(including human hepatocytes). These are the same three groups found when
IRESes were classified according to their activitiesin vitro, or according
to sequence homologies. In a mouse neuronal cell line, the poliovirus and
other type I IRESes were not functional in an artificial bicistronic
context. However, infectious poliovirions were produced efficiently after
transfection of these cells with a genomic length RNA. Furthermore,
activity of the type I IRESes was dramatically increased upon co-expression
of the poliovirus 2A proteinase, demonstrating that while IRES efficiency
may vary considerably from one cell type to another, at least in some cases
viral proteins are capable of overcoming cell-specific translational
defects.
ARTICLES
Comparison of picornaviral IRES-driven internal initiation of translation in cultured cells of different origins
Unite de Virologie Moleculaire (CNRS URA 1966) and 1 Laboratoire des Lyssavirus, Institut Pasteur, 25, rue du Dr Roux, 75724 Paris cedex 15, France.
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