Nucleic Acids Research, Vol 25, Issue 5 992-994, Copyright © 1997 by Oxford University Press
W Chaung, LJ Mi and RJ Boorstein
Chinese hamster lung fibroblast V79 cells have been widely used in studies
of DNA damage and DNA repair. Since the p53 gene is involved in normal
responses to DNA damage, we have analyzed the molecular genetics and
functional status of p53 in V79 cells and primary Chinese hamster embryonic
fibroblast (CHEF) cells. The coding product of the p53 gene in CHEF cells
was 76 and 75% homologous to human and mouse p53 respectively, and was 95%
homologous to the Syrian hamster cells. The V79 p53 sequence contained two
point mutations located within a presumed DNA binding domain, as compared
with the CHEF cells. Additional immunocytochemical and molecular studies
confirmed that the p53 protein in V79 cells was mutated and nonfunctional.
Our results indicate that caution should be used in interpreting studies of
DNA damage, DNA repair and apoptosis in V79 cells.
ARTICLES
The p53 status of Chinese hamster V79 cells frequently used for studies on DNA damage and DNA repair
Department of Pathology, New York University School of Medicine, Sackler Institute of Graduate Biomedical Sciences, New York, NY 10016, USA.
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