Nucleic Acids Research, Vol 25, Issue 5 995-1001, Copyright © 1997 by Oxford University Press
D Avni, Y Biberman and O Meyuhas
TOP mRNAs are vertebrate transcripts which contain a 5'terminal
oligopyrimidine tract (5'TOP), encode for ribosomal proteins and elongation
factors 1alpha and 2, and are candidates for growth- dependent
translational control mediated through their 5'TOP. In the present study we
show that elongation factor 2 (EF2) mRNA is translationally regulated in a
growth-dependent manner in cells of hematopoietic origin, but not in any of
three different non- hematopoietic cell lines studied. Human beta1-tubulin
mRNA is a new member of the family which contains all the hallmarks of a
typical TOP mRNA, yet its translation is refractory to growth arrest of any
of the examined cell lines. Transfection experiments indicate that the
first 29 and 53 nucleotides of the mRNAs encoding EF2 and beta1-tubulin,
respectively, contain all the translational cis-regulatory elements
sufficient for ubiquitously conferring growth-dependent translational
control on a reporter mRNA. These results suggest that the distinct
translational regulation of TOP mRNAs reflects downstream sequences which
can override the regulatory features of the 5'TOP in a cell type- specific
manner. This notion is further supported by the fact that mutations within
the region immediately downstream of the 5'TOP of rpS16 mRNA confer onto
the resulting transcripts growth-dependent translational control with a
cell type specificity similar to that displayed by EF2 mRNA.
ARTICLES
The 5' terminal oligopyrimidine tract confers translational control on TOP mRNAs in a cell type- and sequence context-dependent manner
Department of Biochemistry, The Hebrew University Hadassah Medical School, PO Box 12272, Jerusalem 91120, Israel.
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