Nucleic Acids Research, Vol 25, Issue 5 995-1001, Copyright © 1997 by Oxford University Press
D Avni, Y Biberman and O Meyuhas
TOP mRNAs are vertebrate transcripts which contain a 5'terminal
oligopyrimidine tract (5'TOP), encode for ribosomal proteins and elongation
factors 1alpha and 2, and are candidates for growth- dependent
translational control mediated through their 5'TOP. In the present study we
show that elongation factor 2 (EF2) mRNA is translationally regulated in a
growth-dependent manner in cells of hematopoietic origin, but not in any of
three different non- hematopoietic cell lines studied. Human beta1-tubulin
mRNA is a new member of the family which contains all the hallmarks of a
typical TOP mRNA, yet its translation is refractory to growth arrest of any
of the examined cell lines. Transfection experiments indicate that the
first 29 and 53 nucleotides of the mRNAs encoding EF2 and beta1-tubulin,
respectively, contain all the translational cis-regulatory elements
sufficient for ubiquitously conferring growth-dependent translational
control on a reporter mRNA. These results suggest that the distinct
translational regulation of TOP mRNAs reflects downstream sequences which
can override the regulatory features of the 5'TOP in a cell type- specific
manner. This notion is further supported by the fact that mutations within
the region immediately downstream of the 5'TOP of rpS16 mRNA confer onto
the resulting transcripts growth-dependent translational control with a
cell type specificity similar to that displayed by EF2 mRNA.
ARTICLES
The 5' terminal oligopyrimidine tract confers translational control on TOP mRNAs in a cell type- and sequence context-dependent manner
Department of Biochemistry, The Hebrew University Hadassah Medical School, PO Box 12272, Jerusalem 91120, Israel.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  V. Iadevaia, S. Caldarola, E. Tino, F. Amaldi, and F. Loreni All translation elongation factors and the e, f, and h subunits of translation initiation factor 3 are encoded by 5'-terminal oligopyrimidine (TOP) mRNAs RNA, September 1, 2008; 14(9): 1730 - 1736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Farrar, M. Nater, E. Caywood, M. A. McDevitt, J. Kowalski, C. M. Takemoto, C. C. Talbot Jr, P. Meltzer, D. Esposito, A. H. Beggs, et al. Abnormalities of the large ribosomal subunit protein, Rpl35a, in Diamond-Blackfan anemia Blood, September 1, 2008; 112(5): 1582 - 1592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Thomas and G. J. Johannes Identification of mRNAs that continue to associate with polysomes during hypoxia RNA, July 1, 2007; 13(7): 1116 - 1131. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Stolovich, T. Lerer, Y. Bolkier, H. Cohen, and O. Meyuhas Lithium Can Relieve Translational Repression of TOP mRNAs Elicited by Various Blocks along the Cell Cycle in a Glycogen Synthase Kinase-3- and S6-Kinase-independent Manner J. Biol. Chem., February 18, 2005; 280(7): 5336 - 5342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-H. Yu, Y. Zhang, P. Oelkers, S. L. Sturley, D. J. Rader, and H. N. Ginsberg Posttranscriptional Control of the Expression and Function of Diacylglycerol Acyltransferase-1 in Mouse Adipocytes J. Biol. Chem., December 20, 2002; 277(52): 50876 - 50884. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Stolovich, H. Tang, E. Hornstein, G. Levy, R. Cohen, S. S. Bae, M. J. Birnbaum, and O. Meyuhas Transduction of Growth or Mitogenic Signals into Translational Activation of TOP mRNAs Is Fully Reliant on the Phosphatidylinositol 3-Kinase-Mediated Pathway but Requires neither S6K1 nor rpS6 Phosphorylation Mol. Cell. Biol., December 1, 2002; 22(23): 8101 - 8113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Barth-Baus, C. A. Stratton, L. Parrott, H. Myerson, O. Meyuhas, D. J. Templeton, G. E. Landreth, and J. O. Hensold S6 phosphorylation-independent pathways regulate translation of 5'-terminal oligopyrimidine tract-containing mRNAs in differentiating hematopoietic cells Nucleic Acids Res., May 1, 2002; 30(9): 1919 - 1928. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. HORNSTEIN, H. TANG, and O. MEYUHAS Mitogenic and Nutritional Signals Are Transduced into Translational Efficiency of TOP mRNAs Cold Spring Harb Symp Quant Biol, January 1, 2001; 66(0): 477 - 484. [Abstract] [PDF]
|
|
|
|
 |
|
 M. Gale Jr., S.-L. Tan, and M. G. Katze Translational Control of Viral Gene Expression in Eukaryotes Microbiol. Mol. Biol. Rev., June 1, 2000; 64(2): 239 - 280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Hornstein, A. Git, I. Braunstein, D. Avni, and O. Meyuhas The Expression of Poly(A)-binding Protein Gene Is Translationally Regulated in a Growth-dependent Fashion through a 5'-Terminal Oligopyrimidine Tract Motif J. Biol. Chem., January 15, 1999; 274(3): 1708 - 1714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hashemolhosseini, Y. Nagamine, S. J. Morley, S. Desrivieres, L. Mercep, and S. Ferrari Rapamycin Inhibition of the G1 to S Transition Is Mediated by Effects on Cyclin D1 mRNA and Protein Stability J. Biol. Chem., June 5, 1998; 273(23): 14424 - 14429. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. V. Davuluri, Y. Suzuki, S. Sugano, and M. Q. Zhang CART Classification of Human 5' UTR Sequences Genome Res., November 1, 2000; 10(11): 1807 - 1816. [Abstract] [Full Text]
|
|