Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (473K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (114)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Perler, F. B.
Right arrow Articles by Adam, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Perler, F. B.
Right arrow Articles by Adam, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 25, Issue 6 1087-1093, Copyright © 1997 by Oxford University Press

ARTICLES

Compilation and analysis of intein sequences

FB Perler, GJ Olsen and E Adam
New England Biolabs Inc., Beverly, MA 01915, USA. perler@neb.com

We have compiled a list of all the inteins (protein splicing elements) whose sequences have been published or were available from on-line sequence databases as of September 18, 1996. Analysis of the 36 available intein sequences refines the previously described intein motifs and reveals the presence of another intein motif, Block H. Furthermore, analysis of the new inteins reshapes our view of the conserved splice junction residues, since three inteins lack the intein penultimate His seen in prior examples. Comparison of intein sequences suggests that, in general, (i) inteins present in the same location within extein homologs from different organisms are very closely related to each other in paired sequence comparison or phylogenetic analysis and we suggest that they should be considered intein alleles; (ii) multiple inteins present in the same gene are no more similar to each other than to inteins present in different genes; (iii) phylogenetic analysis indicates that inteins are so divergent that trees with statistically significant branches cannot be generated except for intein alleles.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Protein Sci.Home page
M. A. Johnson, M. W. Southworth, T. Herrmann, L. Brace, F. B. Perler, and K. Wuthrich
NMR structure of a KlbA intein precursor from Methanococcus jannaschii
Protein Sci., July 1, 2007; 16(7): 1316 - 1328.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Bakhrat, K. Baranes, O. Krichevsky, I. Rom, G. Schlenstedt, S. Pietrokovski, and D. Raveh
Nuclear Import of Ho Endonuclease Utilizes Two Nuclear Localization Signals and Four Importins of the Ribosomal Import System
J. Biol. Chem., May 5, 2006; 281(18): 12218 - 12226.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
G. Huet, M. Daffe, and I. Saves
Identification of the Mycobacterium tuberculosis SUF Machinery as the Exclusive Mycobacterial System of [Fe-S] Cluster Assembly: Evidence for Its Implication in the Pathogen's Survival
J. Bacteriol., September 1, 2005; 187(17): 6137 - 6146.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
K. Nagasaki, Y. Shirai, Y. Tomaru, K. Nishida, and S. Pietrokovski
Algal Viruses with Distinct Intraspecies Host Specificities Include Identical Intein Elements
Appl. Envir. Microbiol., July 1, 2005; 71(7): 3599 - 3607.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X.-Q. Liu and J. Yang
Split dnaE Genes Encoding Multiple Novel Inteins in Trichodesmium erythraeum
J. Biol. Chem., July 11, 2003; 278(29): 26315 - 26318.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Thion, E. Laurine, M. Erard, O. Burlet-Schiltz, B. Monsarrat, J.-M. Masson, and I. Saves
The Two-step Cleavage Activity of PI-TfuI Intein Endonuclease Demonstrated by Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry
J. Biol. Chem., November 15, 2002; 277(47): 45442 - 45450.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
I. Saves, C. Morlot, L. Thion, J.-L. Rolland, J. Dietrich, and J.-M. Masson
Investigating the endonuclease activity of four Pyrococcus abyssi inteins
Nucleic Acids Res., October 1, 2002; 30(19): 4158 - 4165.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
V. Koufopanou, M. R. Goddard, and A. Burt
Adaptation for Horizontal Transfer in a Homing Endonuclease
Mol. Biol. Evol., March 1, 2002; 19(3): 239 - 246.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
I. Saves, F. Westrelin, M. Daffe, and J.-M. Masson
Identification of the first eubacterial endonuclease coded by an intein allele in the pps1 gene of mycobacteria
Nucleic Acids Res., November 1, 2001; 29(21): 4310 - 4318.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
F. S. Gimble
Degeneration of a homing endonuclease and its target sequence in a wild yeast strain
Nucleic Acids Res., October 15, 2001; 29(20): 4215 - 4223.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. S. Chevalier and B. L. Stoddard
Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility
Nucleic Acids Res., September 15, 2001; 29(18): 3757 - 3774.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
I. Saves, H. Eleaume, J. Dietrich, and J.-M. Masson
The Thy Pol-2 intein of Thermococcus hydrothermalis is an isoschizomer of PI-TliI and PI-TfuII endonucleases
Nucleic Acids Res., November 1, 2000; 28(21): 4391 - 4396.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K. Frischkorn, B. Springer, E. C. Böttger, E. O. Davis, M. J. Colston, and P. Sander
In Vivo Splicing and Functional Characterization of Mycobacterium leprae RecA
J. Bacteriol., June 15, 2000; 182(12): 3590 - 3592.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
B. W. Poland, M.-Q. Xu, and F. A. Quiocho
Structural Insights into the Protein Splicing Mechanism of PI-SceI
J. Biol. Chem., May 26, 2000; 275(22): 16408 - 16413.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Saves, V. Ozanne, J. Dietrich, and J.-M. Masson
Inteins of Thermococcus fumicolans DNA Polymerase Are Endonucleases with Distinct Enzymatic Behaviors
J. Biol. Chem., January 28, 2000; 275(4): 2335 - 2341.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. P. Scott, E. Abel-Santos, M. Wall, D. C. Wahnon, and S. J. Benkovic
Production of cyclic peptides and proteins in vivo
PNAS, November 23, 1999; 96(24): 13638 - 13643.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
G. Aspöck, H. Kagoshima, G. Niklaus, and T. R. Bürglin
Caenorhabditis elegans Has Scores of hedgehogRelated Genes: Sequence and Expression Analysis
Genome Res., October 1, 1999; 9(10): 909 - 923.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. W. Hendrix, M. C. M. Smith, R. N. Burns, M. E. Ford, and G. F. Hatfull
Evolutionary relationships among diverse bacteriophages and prophages: All the world's a phage
PNAS, March 2, 1999; 96(5): 2192 - 2197.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. S. Gimble, X. Duan, D. Hu, and F. A. Quiocho
Identification of Lys-403 in the PI-SceI Homing Endonuclease as Part of a Symmetric Catalytic Center
J. Biol. Chem., November 13, 1998; 273(46): 30524 - 30529.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Wu, Z. Hu, and X.-Q. Liu
Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803
PNAS, August 4, 1998; 95(16): 9226 - 9231.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. M. Lew, K. V. Mills, and H. Paulus
Protein Splicing in Vitro with a Semisynthetic Two-component Minimal Intein
J. Biol. Chem., June 26, 1998; 273(26): 15887 - 15890.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Chong, K. S. Williams, C. Wotkowicz, and M.-Q. Xu
Modulation of Protein Splicing of the Saccharomyces cerevisiae Vacuolar Membrane ATPase Intein
J. Biol. Chem., April 24, 1998; 273(17): 10567 - 10577.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. He, M. Crist, H.-c. Yen, X. Duan, F. A. Quiocho, and F. S. Gimble
Amino Acid Residues in Both the Protein Splicing and Endonuclease Domains of the PI-SceI Intein Mediate DNA Binding
J. Biol. Chem., February 20, 1998; 273(8): 4607 - 4615.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. Derbyshire and M. Belfort
Lightning strikes twice: Intron-intein coincidence
PNAS, February 17, 1998; 95(4): 1356 - 1357.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. Lazarevic, B. Soldo, A. Dusterhoft, H. Hilbert, C. Mauel, and D. Karamata
Introns and intein coding sequence in the ribonucleotide reductase genes of Bacillus subtilis temperate bacteriophage SPbeta
PNAS, February 17, 1998; 95(4): 1692 - 1697.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
P Sander, F Alcaide, I Richter, K Frischkorn, E Tortoli, B Springer, A Telenti, and E. Bottger
Inteins in mycobacterial GyrA are a taxonomic character
Microbiology, February 1, 1998; 144(2): 589 - 591.
[Abstract]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. Derbyshire, D. W. Wood, W. Wu, J. T. Dansereau, J. Z. Dalgaard, and M. Belfort
Genetic definition of a protein-splicing domain: Functional mini-inteins support structure predictions and a model for intein evolution
PNAS, October 14, 1997; 94(21): 11466 - 11471.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
X.-Q. Liu and Z. Hu
A DnaB intein in Rhodothermus marinus: Indication of recent intein homing across remotely related organisms
PNAS, July 22, 1997; 94(15): 7851 - 7856.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Chong and M.-Q. Xu
Protein Splicing of the Saccharomyces cerevisiae VMA Intein without the Endonuclease Motifs
J. Biol. Chem., June 20, 1997; 272(25): 15587 - 15590.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Chen, J. Benner, and F. B. Perler
Protein Splicing in the Absence of an Intein Penultimate Histidine
J. Biol. Chem., June 30, 2000; 275(27): 20431 - 20435.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Ghosh, L. Sun, and M.-Q. Xu
Zinc Inhibition of Protein trans-Splicing and Identification of Regions Essential for Splicing and Association of a Split Intein*
J. Biol. Chem., June 22, 2001; 276(26): 24051 - 24058.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.