Nucleic Acids Research, Vol 25, Issue 6 1148-1154, Copyright © 1997 by Oxford University Press
M Nashimoto
Mammalian tRNA 3'processing endoribonuclease (3'tRNase) removes 3'extra
nucleotides after the discriminator from tRNA precursors. Here I examined
how the length of a 3'trailer and the nucleotides on each side of the
cleavage site affected 3'processing efficiency. I performed in vitro
3'processing reactions of pre-tRNAArgs with various 3'trailers or various
discriminator nucleotides using 3'tRNase purified from mouse FM3A cells or
pig liver. On the whole, the efficiency of pre- tRNAArg3'processing by
mammalian 3'tRNase decreased as the 3'trailer became longer, except in the
case of a 3'trailer composed of CC, CCA or CCA plus 1 or 2 nucleotides,
which was not able to be removed at all. The distribution of 3'trailer
lengths deduced from mammalian nuclear tRNA genomic sequences reflects this
property of 3'tRNase. The cleavage efficiency of pre-tRNAArgs varied
depending on the 5'end nucleotide of a 3'trailer in the order G
approximately A > U > C. This effect of the 5'end nucleotide was
independent of the discriminator nucleotides. The distribution of the 5'end
nucleotides of mammalian pre-tRNA 3'trailers reflects this differential
3'processing efficiency.
ARTICLES
Distribution of both lengths and 5' terminal nucleotides of mammalian pre-tRNA 3' trailers reflects properties of 3' processing endoribonuclease
Life Science Research Laboratory, Japan Tobacco Inc., 6-2 Umegaoka, Aoba-ku, Yokohama, Kanagawa 227, Japan. mnashimoto@chemgate.byu.edu
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