Nucleic Acids Research, Vol 25, Issue 6 1162-1169, Copyright © 1997 by Oxford University Press
M Shayeghi, CL Doe, M Tavassoli and FZ Watts
The fission yeast rad31-1 mutant is sensitive to both UV and ionising
radiation and exhibits a growth defect at 35 degrees C. In addition, the
mutant displays defects in cell morphology and nuclear division at 26
degrees C which are exaggerated at 35 degrees C. We have cloned the rad31
gene and have shown that it is not essential for viability, although cells
containing a disrupted rad31 gene grow slowly. The null allele has similar
cell and nuclear morphologies to the original allele and displays an
extremely high frequency of loss of minichromosomes. rad31 is not required
for either the S/M or G2/M checkpoint, however double mutant analysis
indicates that rad31 acts in a process which is defective in the checkpoint
rad mutants and which involves hus5 . Sequence analysis indicates that
rad31 encodes a protein which is related to ubiquitin activating proteins
and more particularly to an ORF in Saccharomyces cerevisiae and to the
Arabidopsis thaliana AXR1 and human APP-BP1 genes. We have isolated the
S.cerevisiae sequence, which we have named RHC31 ( ad31homologue in S.
erevisiae), since we show that it can complement the slow growth phenotype
and radiation sensitivity of S.pombe rad31.
ARTICLES
Characterisation of Schizosaccharomyces pombe rad31, a UBA-related gene required for DNA damage tolerance
Biochemistry, School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK.
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