Nucleic Acids Research, Vol 25, Issue 6 1203-1210, Copyright © 1997 by Oxford University Press
R Lutz and H Bujard
Based on parameters governing promoter activity and using regulatory
elements of the lac, ara and tet operon transcription control sequences
were composed which permit the regulation in Escherichia coli of several
gene activities independently and quantitatively. The novel promoter
PLtetO-1 allows the regulation of gene expression over an up to 5000-fold
range with anhydrotetracycline (aTc) whereas with IPTG and arabinose the
activity of Plac/ara-1 may be controlled 1800-fold. Escherichia coli host
strains which produce defined amounts of the regulatory proteins, Lac and
Tet repressor as well as AraC from chromosomally located expression units
provide highly reproducible in vivo conditions. Controlling the expression
of the genes encoding luciferase, the low abundance E.coli protein DnaJ and
restriction endonuclease Cfr9I not only demonstrates that high levels of
expression can be achieved but also suggests that under conditions of
optimal repression only around one mRNA every 3rd generation is produced.
This potential of quantitative control will open up new approaches in the
study of gene function in vivo, in particular with low abundance regulatory
gene products. The system will also provide new opportunities for the
controlled expression of heterologous genes.
ARTICLES
Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements
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I.-N. Wang, J. Deaton, and R. Young Sizing the Holin Lesion with an Endolysin-{beta}-Galactosidase Fusion J. Bacteriol., February 1, 2003; 185(3): 779 - 787. [Abstract] [Full Text] [PDF]
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O. Kobiler, S. Koby, D. Teff, D. Court, and A. B. Oppenheim The phage lambda CII transcriptional activator carries a C-terminal domain signaling for rapid proteolysis PNAS, November 12, 2002; 99(23): 14964 - 14969. [Abstract] [Full Text] [PDF]
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M. B. Elowitz, A. J. Levine, E. D. Siggia, and P. S. Swain Stochastic Gene Expression in a Single Cell Science, August 16, 2002; 297(5584): 1183 - 1186. [Abstract] [Full Text] [PDF]
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C. A. White-Ziegler, A. M. Black, S. H. Eliades, S. Young, and K. Porter The N-Acetyltransferase RimJ Responds to Environmental Stimuli To Repress pap Fimbrial Transcription in Escherichia coli J. Bacteriol., August 15, 2002; 184(16): 4334 - 4342. [Abstract] [Full Text] [PDF]
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A. L. Beeston and M. G. Surette pfs-Dependent Regulation of Autoinducer 2 Production in Salmonella enterica Serovar Typhimurium J. Bacteriol., July 1, 2002; 184(13): 3450 - 3456. [Abstract] [Full Text] [PDF]
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R. M. T. de Wildt, I. M. Tomlinson, J. L. Ong, and P. Holliger Isolation of receptor-ligand pairs by capture of long-lived multivalent interaction complexes PNAS, June 25, 2002; 99(13): 8530 - 8535. [Abstract] [Full Text] [PDF]
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C&a.;l. C. Guet, M. B. Elowitz, W. Hsing, and S. Leibler Combinatorial Synthesis of Genetic Networks Science, May 24, 2002; 296(5572): 1466 - 1470. [Abstract] [Full Text] [PDF]
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J. Nesper, A. Krai{beta}, S. Schild, J. Bla{beta}, K. E. Klose, J. Bockemuhl, and J. Reidl Comparative and Genetic Analyses of the Putative Vibrio cholerae Lipopolysaccharide Core Oligosaccharide Biosynthesis (wav) Gene Cluster Infect. Immun., May 1, 2002; 70(5): 2419 - 2433. [Abstract] [Full Text] [PDF]
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F. Qian and W. Pan Construction of a tetR-Integrated Salmonella enterica Serovar Typhi CVD908 Strain That Tightly Controls Expression of the Major Merozoite Surface Protein of Plasmodium falciparum for Applications in Human Vaccine Production Infect. Immun., April 1, 2002; 70(4): 2029 - 2038. [Abstract] [Full Text] [PDF]
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J. C. Chen, M. Minev, and J. Beckwith Analysis of ftsQ Mutant Alleles in Escherichia coli: Complementation, Septal Localization, and Recruitment of Downstream Cell Division Proteins J. Bacteriol., February 1, 2002; 184(3): 695 - 705. [Abstract] [Full Text] [PDF]
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A. Wentzel, A. Christmann, T. Adams, and H. Kolmar Display of Passenger Proteins on the Surface of Escherichia coli K-12 by the Enterohemorrhagic E. coli Intimin EaeA J. Bacteriol., December 15, 2001; 183(24): 7273 - 7284. [Abstract] [Full Text] [PDF]
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J. H. J. Leveau and S. E. Lindow Predictive and Interpretive Simulation of Green Fluorescent Protein Expression in Reporter Bacteria J. Bacteriol., December 1, 2001; 183(23): 6752 - 6762. [Abstract] [Full Text] [PDF]
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D. A. Schofield, C. Westwater, J. W. Dolan, M. G. Schmidt, and J. S. Norris Controlled Expression in Klebsiella pneumoniae and Shigella flexneri Using a Bacteriophage P1-Derived C1-Regulated Promoter System J. Bacteriol., December 1, 2001; 183(23): 6947 - 6950. [Abstract] [Full Text] [PDF]
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