Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (229K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (272)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lutz, R.
Right arrow Articles by Bujard, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lutz, R.
Right arrow Articles by Bujard, H.
Social Bookmarking
 Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 25, Issue 6 1203-1210, Copyright © 1997 by Oxford University Press


ARTICLES

Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements

R Lutz and H Bujard
ZMBH Zentrum fur Molekulare Biologie der Universitat Heidelberg, Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany.

Based on parameters governing promoter activity and using regulatory elements of the lac, ara and tet operon transcription control sequences were composed which permit the regulation in Escherichia coli of several gene activities independently and quantitatively. The novel promoter PLtetO-1 allows the regulation of gene expression over an up to 5000-fold range with anhydrotetracycline (aTc) whereas with IPTG and arabinose the activity of Plac/ara-1 may be controlled 1800-fold. Escherichia coli host strains which produce defined amounts of the regulatory proteins, Lac and Tet repressor as well as AraC from chromosomally located expression units provide highly reproducible in vivo conditions. Controlling the expression of the genes encoding luciferase, the low abundance E.coli protein DnaJ and restriction endonuclease Cfr9I not only demonstrates that high levels of expression can be achieved but also suggests that under conditions of optimal repression only around one mRNA every 3rd generation is produced. This potential of quantitative control will open up new approaches in the study of gene function in vivo, in particular with low abundance regulatory gene products. The system will also provide new opportunities for the controlled expression of heterologous genes.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
J. Bacteriol.Home page
V. J. Carabetta, B. K. Mohanty, S. R. Kushner, and T. J. Silhavy
The Response Regulator SprE (RssB) Modulates Polyadenylation and mRNA Stability in Escherichia coli
J. Bacteriol., November 15, 2009; 191(22): 6812 - 6821.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
N. Said, R. Rieder, R. Hurwitz, J. Deckert, H. Urlaub, and J. Vogel
In vivo expression and purification of aptamer-tagged small RNA regulators
Nucleic Acids Res., November 1, 2009; 37(20): e133 - e133.
[Abstract] [Full Text] [PDF]


Home page
Appl. Environ. Microbiol.Home page
C. R. Whetstine, J. G. Slusser, and W. R. Zuckert
Development of a Single-Plasmid-Based Regulatable Gene Expression System for Borrelia burgdorferi
Appl. Envir. Microbiol., October 15, 2009; 75(20): 6553 - 6558.
[Abstract] [Full Text] [PDF]


Home page
Infect. Immun.Home page
S. C. F. Sampaio, T. A. T. Gomes, C. Pichon, L. du Merle, S. Guadagnini, C. M. Abe, J. L. M. Sampaio, and C. Le Bouguenec
The Flagella of an Atypical Enteropathogenic Escherichia coli Strain Are Required for Efficient Interaction with and Stimulation of Interleukin-8 Production by Enterocytes In Vitro
Infect. Immun., October 1, 2009; 77(10): 4406 - 4413.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
J. Oberto, N. Breuil, A. Hecker, F. Farina, C. Brochier-Armanet, E. Culetto, and P. Forterre
Qri7/OSGEPL, the mitochondrial version of the universal Kae1/YgjD protein, is essential for mitochondrial genome maintenance
Nucleic Acids Res., September 1, 2009; 37(16): 5343 - 5352.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
C. S. Wadler and C. K. Vanderpool
Characterization of homologs of the small RNA SgrS reveals diversity in function
Nucleic Acids Res., September 1, 2009; 37(16): 5477 - 5485.
[Abstract] [Full Text] [PDF]


Home page
MicrobiologyHome page
M. D. Mastropaolo, M. L. Thorson, and A. M. Stevens
Comparison of Bacteroides thetaiotaomicron and Escherichia coli 16S rRNA gene expression signals
Microbiology, August 1, 2009; 155(8): 2683 - 2693.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
M. Dehbi, G. Moeck, F. F. Arhin, P. Bauda, D. Bergeron, T. Kwan, J. Liu, J. McCarty, M. DuBow, and J. Pelletier
Inhibition of Transcription in Staphylococcus aureus by a Primary Sigma Factor-Binding Polypeptide from Phage G1
J. Bacteriol., June 15, 2009; 191(12): 3763 - 3771.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
S. Saini, J. A. Pearl, and C. V. Rao
Role of FimW, FimY, and FimZ in Regulating the Expression of Type I Fimbriae in Salmonella enterica Serovar Typhimurium
J. Bacteriol., May 1, 2009; 191(9): 3003 - 3010.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
D. Garcia-Moreno, M. C. Polanco, G. Navarro-Aviles, F. J. Murillo, S. Padmanabhan, and M. Elias-Arnanz
A Vitamin B12-Based System for Conditional Expression Reveals dksA To Be an Essential Gene in Myxococcus xanthus
J. Bacteriol., May 1, 2009; 191(9): 3108 - 3119.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
M. Klotzsche, S. Ehrt, and D. Schnappinger
Improved tetracycline repressors for gene silencing in mycobacteria
Nucleic Acids Res., April 1, 2009; 37(6): 1778 - 1788.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
T. K. Lu and J. J. Collins
Engineered bacteriophage targeting gene networks as adjuvants for antibiotic therapy
PNAS, March 24, 2009; 106(12): 4629 - 4634.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
S. Wegener-Feldbrugge and L. Sogaard-Andersen
The Atypical Hybrid Histidine Protein Kinase RodK in Myxococcus xanthus: Spatial Proximity Supersedes Kinetic Preference in Phosphotransfer Reactions
J. Bacteriol., March 15, 2009; 191(6): 1765 - 1776.
[Abstract] [Full Text] [PDF]


Home page
Appl. Environ. Microbiol.Home page
S. Atsumi and J. C. Liao
Directed Evolution of Methanococcus jannaschii Citramalate Synthase for Biosynthesis of 1-Propanol and 1-Butanol by Escherichia coli
Appl. Envir. Microbiol., December 15, 2008; 74(24): 7802 - 7808.
[Abstract] [Full Text] [PDF]


Home page
Appl. Environ. Microbiol.Home page
F. Lakhal, S. Bury-Mone, Y. Nomane, N. Le Goic, C. Paillard, and A. Jacq
DjlA, a Membrane-Anchored DnaJ-Like Protein, Is Required for Cytotoxicity of Clam Pathogen Vibrio tapetis to Hemocytes
Appl. Envir. Microbiol., September 15, 2008; 74(18): 5750 - 5758.
[Abstract] [Full Text] [PDF]


Home page
Appl. Environ. Microbiol.Home page
M. R. Connor and J. C. Liao
Engineering of an Escherichia coli Strain for the Production of 3-Methyl-1-Butanol
Appl. Envir. Microbiol., September 15, 2008; 74(18): 5769 - 5775.
[Abstract] [Full Text] [PDF]


Home page
JCBHome page
M. Bogdanov, J. Xie, P. Heacock, and W. Dowhan
To flip or not to flip: lipid-protein charge interactions are a determinant of final membrane protein topology
J. Cell Biol., September 9, 2008; 182(5): 925 - 935.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
M.-H. Mazauric, P. Licznar, M.-F. Prere, I. Canal, and O. Fayet
Apical Loop-Internal Loop RNA Pseudoknots: A NEW TYPE OF STIMULATOR OF-1 TRANSLATIONAL FRAMESHIFTING IN BACTERIA
J. Biol. Chem., July 18, 2008; 283(29): 20421 - 20432.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
S. Saini, J. D. Brown, P. D. Aldridge, and C. V. Rao
FliZ Is a Posttranslational Activator of FlhD4C2-Dependent Flagellar Gene Expression
J. Bacteriol., July 15, 2008; 190(14): 4979 - 4988.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
C. C. Guet, L. Bruneaux, T. L. Min, D. Siegal-Gaskins, I. Figueroa, T. Emonet, and P. Cluzel
Minimally invasive determination of mRNA concentration in single living bacteria
Nucleic Acids Res., July 1, 2008; 36(12): e73 - e73.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
L. M. Chubiz and C. V. Rao
Computational design of orthogonal ribosomes
Nucleic Acids Res., July 1, 2008; 36(12): 4038 - 4046.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
O. Gefen, C. Gabay, M. Mumcuoglu, G. Engel, and N. Q. Balaban
Single-cell protein induction dynamics reveals a period of vulnerability to antibiotics in persister bacteria
PNAS, April 22, 2008; 105(16): 6145 - 6149.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
J. Valle, S. Da Re, S. Schmid, D. Skurnik, R. D'Ari, and J.-M. Ghigo
The Amino Acid Valine Is Secreted in Continuous-Flow Bacterial Biofilms
J. Bacteriol., January 1, 2008; 190(1): 264 - 274.
[Abstract] [Full Text] [PDF]


Home page
Appl. Environ. Microbiol.Home page
T. Hanai, S. Atsumi, and J. C. Liao
Engineered Synthetic Pathway for Isopropanol Production in Escherichia coli
Appl. Envir. Microbiol., December 15, 2007; 73(24): 7814 - 7818.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
G. Monderer-Rothkoff and O. Amster-Choder
Genetic Dissection of the Divergent Activities of the Multifunctional Membrane Sensor BglF
J. Bacteriol., December 1, 2007; 189(23): 8601 - 8615.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
T. A. T. Tran, D. K. Struck, and R. Young
The T4 RI Antiholin Has an N-Terminal Signal Anchor Release Domain That Targets It for Degradation by DegP
J. Bacteriol., November 1, 2007; 189(21): 7618 - 7625.
[Abstract] [Full Text] [PDF]


Home page
Genes Dev.Home page
C. M. Sharma, F. Darfeuille, T. H. Plantinga, and J. Vogel
A small RNA regulates multiple ABC transporter mRNAs by targeting C/A-rich elements inside and upstream of ribosome-binding sites
Genes & Dev., November 1, 2007; 21(21): 2804 - 2817.
[Abstract] [Full Text] [PDF]


Home page
Genes Dev.Home page
R. A. Schubert, I. B. Dodd, J. B. Egan, and K. E. Shearwin
Cro's role in the CI Cro bistable switch is critical for {lambda}'s transition from lysogeny to lytic development
Genes & Dev., October 1, 2007; 21(19): 2461 - 2472.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
M. N. Win and C. D. Smolke
From the Cover: A modular and extensible RNA-based gene-regulatory platform for engineering cellular function
PNAS, September 4, 2007; 104(36): 14283 - 14288.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
U. Mechold, G. Fang, S. Ngo, V. Ogryzko, and A. Danchin
YtqI from Bacillus subtilis has both oligoribonuclease and pAp-phosphatase activity
Nucleic Acids Res., July 26, 2007; 35(13): 4552 - 4561.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
J. R. Bernstein, T. Bulter, C. R. Shen, and J. C. Liao
Directed Evolution of Ribosomal Protein S1 for Enhanced Translational Efficiency of High GC Rhodopseudomonas palustris DNA in Escherichia coli
J. Biol. Chem., June 29, 2007; 282(26): 18929 - 18936.
[Abstract] [Full Text] [PDF]


Home page
Appl. Environ. Microbiol.Home page
S. Da Re, B. Le Quere, J.-M. Ghigo, and C. Beloin
Tight Modulation of Escherichia coli Bacterial Biofilm Formation through Controlled Expression of Adhesion Factors
Appl. Envir. Microbiol., May 15, 2007; 73(10): 3391 - 3403.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
T. Kuhlman, Z. Zhang, M. H. Saier Jr., and T. Hwa
Combinatorial transcriptional control of the lactose operon of Escherichia coli
PNAS, April 3, 2007; 104(14): 6043 - 6048.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
K. I. Udekwu and E. G. H. Wagner
Sigma E controls biogenesis of the antisense RNA MicA
Nucleic Acids Res., February 28, 2007; 35(4): 1279 - 1288.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
J. H. Urban and J. Vogel
Translational control and target recognition by Escherichia coli small RNAs in vivo
Nucleic Acids Res., February 16, 2007; 35(3): 1018 - 1037.
[Abstract] [Full Text] [PDF]


Home page
ScienceHome page
W. W. Lathem, P. A. Price, V. L. Miller, and W. E. Goldman
A Plasminogen-Activating Protease Specifically Controls the Development of Primary Pneumonic Plague
Science, January 26, 2007; 315(5811): 509 - 513.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
M. Kawe, P. Forrer, P. Amstutz, and A. Pluckthun
Isolation of Intracellular Proteinase Inhibitors Derived from Designed Ankyrin Repeat Proteins by Genetic Screening
J. Biol. Chem., December 29, 2006; 281(52): 40252 - 40263.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
C. W. Kauth, U. Woehlbier, M. Kern, Z. Mekonnen, R. Lutz, N. Mucke, J. Langowski, and H. Bujard
Interactions between Merozoite Surface Proteins 1, 6, and 7 of the Malaria Parasite Plasmodium falciparum
J. Biol. Chem., October 20, 2006; 281(42): 31517 - 31527.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
A. Hsiao, Z. Liu, A. Joelsson, and J. Zhu
Vibrio cholerae virulence regulator-coordinated evasion of host immunity
PNAS, September 26, 2006; 103(39): 14542 - 14547.
[Abstract] [Full Text] [PDF]


Home page
Appl. Environ. Microbiol.Home page
M. Schlicht, C. Berens, J. Daam, and W. Hillen
Random Insertion of a TetR-Inducing Peptide Tag into Escherichia coli Proteins Allows Analysis of Protein Levels by Induction of Reporter Gene Expression
Appl. Envir. Microbiol., August 1, 2006; 72(8): 5637 - 5642.
[Abstract] [Full Text] [PDF]


Home page
Appl. Environ. Microbiol.Home page
K. Jensen, H. Alper, C. Fischer, and G. Stephanopoulos
Identifying functionally important mutations from phenotypically diverse sequence data.
Appl. Envir. Microbiol., May 1, 2006; 72(5): 3696 - 3701.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
S. Da Re and J.-M. Ghigo
A CsgD-Independent Pathway for Cellulose Production and Biofilm Formation in Escherichia coli.
J. Bacteriol., April 1, 2006; 188(8): 3073 - 3087.
[Abstract] [Full Text] [PDF]


Home page
Genes Dev.Home page
A. Bougdour, S. Wickner, and S. Gottesman
Modulating RssB activity: IraP, a novel regulator of {sigma}S stability in Escherichia coli.
Genes & Dev., April 1, 2006; 20(7): 884 - 897.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
L. Dubytska, H. P. Godfrey, and F. C. Cabello
Borrelia burgdorferi ftsZ Plays a Role in Cell Division.
J. Bacteriol., March 1, 2006; 188(5): 1969 - 1978.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
P. Srivastava, R. A. Fekete, and D. K. Chattoraj
Segregation of the Replication Terminus of the Two Vibrio cholerae Chromosomes
J. Bacteriol., February 1, 2006; 188(3): 1060 - 1070.
[Abstract] [Full Text] [PDF]


Home page
Appl. Environ. Microbiol.Home page
T. Lutke-Eversloh and G. Stephanopoulos
Feedback Inhibition of Chorismate Mutase/Prephenate Dehydrogenase (TyrA) of Escherichia coli: Generation and Characterization of Tyrosine-Insensitive Mutants
Appl. Envir. Microbiol., November 1, 2005; 71(11): 7224 - 7228.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
J. L. Royo, H. Manyani, A. Cebolla, and E. Santero
A new generation of vectors with increased induction ratios by overimposing a second regulatory level by attenuation
Nucleic Acids Res., October 31, 2005; 33(19): e169 - e169.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
T. A. T. Tran, D. K. Struck, and R. Young
Periplasmic Domains Define Holin-Antiholin Interactions in T4 Lysis Inhibition
J. Bacteriol., October 1, 2005; 187(19): 6631 - 6640.
[Abstract] [Full Text] [PDF]


Home page
Genes Dev.Home page
K. I. Udekwu, F. Darfeuille, J. Vogel, J. Reimegard, E. Holmqvist, and E. G. H. Wagner
Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA
Genes & Dev., October 1, 2005; 19(19): 2355 - 2366.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
H. Alper, C. Fischer, E. Nevoigt, and G. Stephanopoulos
Tuning genetic control through promoter engineering
PNAS, September 6, 2005; 102(36): 12678 - 12683.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
N. E. Buchler, U. Gerland, and T. Hwa
Nonlinear protein degradation and the function of genetic circuits
PNAS, July 5, 2005; 102(27): 9559 - 9564.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
S. C. J. De Keersmaecker, K. Marchal, T. L. A. Verhoeven, K. Engelen, J. Vanderleyden, and C. S. Detweiler
Microarray Analysis and Motif Detection Reveal New Targets of the Salmonella enterica Serovar Typhimurium HilA Regulatory Protein, Including hilA Itself
J. Bacteriol., July 1, 2005; 187(13): 4381 - 4391.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
M. Merdanovic, E. Sauer, and J. Reidl
Coupling of NAD+ Biosynthesis and Nicotinamide Ribosyl Transport: Characterization of NadR Ribonucleotide Kinase Mutants of Haemophilus influenzae
J. Bacteriol., July 1, 2005; 187(13): 4410 - 4420.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
T. T. Le, S. Harlepp, C. C. Guet, K. Dittmar, T. Emonet, T. Pan, and P. Cluzel
Real-time RNA profiling within a single bacterium
PNAS, June 28, 2005; 102(26): 9160 - 9164.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
K. Zhao, M. Liu, and R. R. Burgess
The Global Transcriptional Response of Escherichia coli to Induced {sigma}32 Protein Involves {sigma}32 Regulon Activation Followed by Inactivation and Degradation of {sigma}32 in Vivo
J. Biol. Chem., May 6, 2005; 280(18): 17758 - 17768.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
C. A. Roessner, H. J. Williams, and A. I. Scott
Genetically Engineered Production of 1-Desmethylcobyrinic Acid, 1-Desmethylcobyrinic Acid a,c-Diamide, and Cobyrinic Acid a,c-Diamide in Escherichia coli Implies a Role for CbiD in C-1 Methylation in the Anaerobic Pathway to Cobalamin
J. Biol. Chem., April 29, 2005; 280(17): 16748 - 16753.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
S. Hooshangi, S. Thiberge, and R. Weiss
Ultrasensitivity and noise propagation in a synthetic transcriptional cascade
PNAS, March 8, 2005; 102(10): 3581 - 3586.
[Abstract] [Full Text] [PDF]


Home page
Appl. Environ. Microbiol.Home page
A. Kamionka, R. Bertram, and W. Hillen
Tetracycline-Dependent Conditional Gene Knockout in Bacillus subtilis
Appl. Envir. Microbiol., February 1, 2005; 71(2): 728 - 733.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
S. Ehrt, X. V. Guo, C. M. Hickey, M. Ryou, M. Monteleone, L. W. Riley, and D. Schnappinger
Controlling gene expression in mycobacteria with anhydrotetracycline and Tet repressor
Nucleic Acids Res., February 1, 2005; 33(2): e21 - e21.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
T. M. Adams, A. Wentzel, and H. Kolmar
Intimin-Mediated Export of Passenger Proteins Requires Maintenance of a Translocation-Competent Conformation
J. Bacteriol., January 15, 2005; 187(2): 522 - 533.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
J. Collier, C. Bohn, and P. Bouloc
SsrA Tagging of Escherichia coli SecM at Its Translation Arrest Sequence
J. Biol. Chem., December 24, 2004; 279(52): 54193 - 54201.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
L. Fux, A. Nussbaum-Shochat, L. Lopian, and O. Amster-Choder
Modulation of Monomer Conformation of the BglG Transcriptional Antiterminator from Escherichia coli
J. Bacteriol., October 15, 2004; 186(20): 6775 - 6781.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
N. C. Martinez-Gomez, M. Robers, and D. M. Downs
Mutational Analysis of ThiH, a Member of the Radical S-Adenosylmethionine (AdoMet) Protein Superfamily
J. Biol. Chem., September 24, 2004; 279(39): 40505 - 40510.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
I. Golding and E. C. Cox
RNA dynamics in live Escherichia coli cells
PNAS, August 3, 2004; 101(31): 11310 - 11315.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
A. Kamionka, M. Sehnal, O. Scholz, and W. Hillen
Independent Regulation of Two Genes in Escherichia coli by Tetracyclines and Tet Repressor Variants
J. Bacteriol., July 1, 2004; 186(13): 4399 - 4401.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
H. Kobayashi, M. Kaern, M. Araki, K. Chung, T. S. Gardner, C. R. Cantor, and J. J. Collins
Programmable cells: Interfacing natural and engineered gene networks
PNAS, June 1, 2004; 101(22): 8414 - 8419.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
N. A. Grieshaber, E. R. Fischer, D. J. Mead, C. A. Dooley, and T. Hackstadt
From The Cover: Chlamydial histone-DNA interactions are disrupted by a metabolite in the methylerythritol phosphate pathway of isoprenoid biosynthesis
PNAS, May 11, 2004; 101(19): 7451 - 7456.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
M. Xu, D. K. Struck, J. Deaton, I.-N. Wang, and R. Young
A signal-arrest-release sequence mediates export and control of the phage P1 endolysin
PNAS, April 27, 2004; 101(17): 6415 - 6420.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
M. C. Saraf, A. R. Horswill, S. J. Benkovic, and C. D. Maranas
From the Cover: FamClash: A method for ranking the activity of engineered enzymes
PNAS, March 23, 2004; 101(12): 4142 - 4147.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
M. D. Wolfe, F. Ahmed, G. M. Lacourciere, C. T. Lauhon, T. C. Stadtman, and T. J. Larson
Functional Diversity of the Rhodanese Homology Domain: THE ESCHERICHIA COLI ybbB GENE ENCODES A SELENOPHOSPHATE-DEPENDENT tRNA 2-SELENOURIDINE SYNTHASE
J. Biol. Chem., January 16, 2004; 279(3): 1801 - 1809.
[Abstract] [Full Text] [PDF]


Home page
Appl. Environ. Microbiol.Home page
I. Hautefort, M. J. Proenca, and J. C. D. Hinton
Single-Copy Green Fluorescent Protein Gene Fusions Allow Accurate Measurement of Salmonella Gene Expression In Vitro and during Infection of Mammalian Cells
Appl. Envir. Microbiol., December 1, 2003; 69(12): 7480 - 7491.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
D. Christ and G. Winter
Identification of functional similarities between proteins using directed evolution
PNAS, November 11, 2003; 100(23): 13202 - 13206.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
T. Tomoyasu, A. Takaya, T. Sasaki, T. Nagase, R. Kikuno, M. Morioka, and T. Yamamoto
A New Heat Shock Gene, agsA, Which Encodes a Small Chaperone Involved in Suppressing Protein Aggregation in Salmonella enterica Serovar Typhimurium
J. Bacteriol., November 1, 2003; 185(21): 6331 - 6339.
[Abstract] [Full Text] [PDF]


Home page
Genome ResHome page
P. M. Kim and B. Tidor
Limitations of Quantitative Gene Regulation Models: A Case Study
Genome Res., November 1, 2003; 13(11): 2391 - 2395.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
J. Bjarnason, C. M. Southward, and M. G. Surette
Genomic Profiling of Iron-Responsive Genes in Salmonella enterica Serovar Typhimurium by High-Throughput Screening of a Random Promoter Library
J. Bacteriol., August 15, 2003; 185(16): 4973 - 4982.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
F. J. Isaacs, J. Hasty, C. R. Cantor, and J. J. Collins
Prediction and measurement of an autoregulatory genetic module
PNAS, June 24, 2003; 100(13): 7714 - 7719.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
C. W. Kauth, C. Epp, H. Bujard, and R. Lutz
The Merozoite Surface Protein 1 Complex of Human Malaria Parasite Plasmodium falciparum: INTERACTIONS AND ARRANGEMENTS OF SUBUNITS
J. Biol. Chem., June 13, 2003; 278(25): 22257 - 22264.
[Abstract] [Full Text] [PDF]


Home page
Appl. Environ. Microbiol.Home page
D. A. Schofield, C. Westwater, B. D. Hoel, P. A. Werner, J. S. Norris, and M. G. Schmidt
Development of a Thermally Regulated Broad-Spectrum Promoter System for Use in Pathogenic Gram-Positive Species
Appl. Envir. Microbiol., June 1, 2003; 69(6): 3385 - 3392.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
I.-N. Wang, J. Deaton, and R. Young
Sizing the Holin Lesion with an Endolysin-{beta}-Galactosidase Fusion
J. Bacteriol., February 1, 2003; 185(3): 779 - 787.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
O. Kobiler, S. Koby, D. Teff, D. Court, and A. B. Oppenheim
The phage lambda CII transcriptional activator carries a C-terminal domain signaling for rapid proteolysis
PNAS, November 12, 2002; 99(23): 14964 - 14969.
[Abstract] [Full Text] [PDF]


Home page
ScienceHome page
M. B. Elowitz, A. J. Levine, E. D. Siggia, and P. S. Swain
Stochastic Gene Expression in a Single Cell
Science, August 16, 2002; 297(5584): 1183 - 1186.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
C. A. White-Ziegler, A. M. Black, S. H. Eliades, S. Young, and K. Porter
The N-Acetyltransferase RimJ Responds to Environmental Stimuli To Repress pap Fimbrial Transcription in Escherichia coli
J. Bacteriol., August 15, 2002; 184(16): 4334 - 4342.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
A. L. Beeston and M. G. Surette
pfs-Dependent Regulation of Autoinducer 2 Production in Salmonella enterica Serovar Typhimurium
J. Bacteriol., July 1, 2002; 184(13): 3450 - 3456.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
R. M. T. de Wildt, I. M. Tomlinson, J. L. Ong, and P. Holliger
Isolation of receptor-ligand pairs by capture of long-lived multivalent interaction complexes
PNAS, June 25, 2002; 99(13): 8530 - 8535.
[Abstract] [Full Text] [PDF]


Home page
ScienceHome page
C&a.;l. C. Guet, M. B. Elowitz, W. Hsing, and S. Leibler
Combinatorial Synthesis of Genetic Networks
Science, May 24, 2002; 296(5572): 1466 - 1470.
[Abstract] [Full Text] [PDF]


Home page
Infect. Immun.Home page
J. Nesper, A. Krai{beta}, S. Schild, J. Bla{beta}, K. E. Klose, J. Bockemuhl, and J. Reidl
Comparative and Genetic Analyses of the Putative Vibrio cholerae Lipopolysaccharide Core Oligosaccharide Biosynthesis (wav) Gene Cluster
Infect. Immun., May 1, 2002; 70(5): 2419 - 2433.
[Abstract] [Full Text] [PDF]


Home page
Infect. Immun.Home page
F. Qian and W. Pan
Construction of a tetR-Integrated Salmonella enterica Serovar Typhi CVD908 Strain That Tightly Controls Expression of the Major Merozoite Surface Protein of Plasmodium falciparum for Applications in Human Vaccine Production
Infect. Immun., April 1, 2002; 70(4): 2029 - 2038.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
J. C. Chen, M. Minev, and J. Beckwith
Analysis of ftsQ Mutant Alleles in Escherichia coli: Complementation, Septal Localization, and Recruitment of Downstream Cell Division Proteins
J. Bacteriol., February 1, 2002; 184(3): 695 - 705.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
A. Wentzel, A. Christmann, T. Adams, and H. Kolmar
Display of Passenger Proteins on the Surface of Escherichia coli K-12 by the Enterohemorrhagic E. coli Intimin EaeA
J. Bacteriol., December 15, 2001; 183(24): 7273 - 7284.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
J. H. J. Leveau and S. E. Lindow
Predictive and Interpretive Simulation of Green Fluorescent Protein Expression in Reporter Bacteria
J. Bacteriol., December 1, 2001; 183(23): 6752 - 6762.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
D. A. Schofield, C. Westwater, J. W. Dolan, M. G. Schmidt, and J. S. Norris
Controlled Expression in Klebsiella pneumoniae and Shigella flexneri Using a Bacteriophage P1-Derived C1-Regulated Promoter System
J. Bacteriol., December 1, 2001; 183(23): 6947 - 6950.
[Abstract] [Full Text] [PDF]



Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.