Nucleic Acids Research, Vol 25, Issue 6 1219-1224, Copyright © 1997 by Oxford University Press
R Oehler, N Polacek, G Steiner and A Barta
Photolysis of [3H]tetracycline in the presence of Escherichia coli
ribosomes results in an approximately 1:1 ratio of labelling ribosomal
proteins and RNAs. In this work we characterize crosslinks to both 16S and
23S RNAs. Previously, the main target of photoincorporation of
[3H]tetracycline into ribosomal proteins was shown to be S7, which is also
part of the one strong binding site of tetracycline on the 30S subunit. The
crosslinks on 23S RNA map exclusively to the central loop of domain V
(G2505, G2576 and G2608) which is part of the peptidyl transferase region.
However, experiments performed with chimeric ribosomal subunits demonstrate
that peptidyltransferase activity is not affected by tetracycline
crosslinked solely to the 50S subunits. Three different positions are
labelled on the 16S RNA, G693, G1300 and G1338. The positions of these
crosslinked nucleotides correlate well with footprints on the 16S RNA
produced either by tRNA or the protein S7. This suggests that the
nucleotides are labelled by tetracycline bound to the strong binding site
on the 30S subunit. In addition, our results demonstrate that the well
known inhibition of tRNA binding to the A- site is solely due to
tetracycline crosslinked to 30S subunits and furthermore suggest that
interactions of the antibiotic with 16S RNA might be involved in its mode
of action.
ARTICLES
Interaction of tetracycline with RNA: photoincorporation into ribosomal RNA of Escherichia coli
Institute of Biochemistry, University of Vienna, Vienna Biocenter, Dr Bohrgasse 9/3, A-1030 Vienna, Austria.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. B. S. Sidhu, Q. Sun, L. J. Nkrumah, M. W. Dunne, J. C. Sacchettini, and D. A. Fidock In Vitro Efficacy, Resistance Selection, and Structural Modeling Studies Implicate the Malarial Parasite Apicoplast as the Target of Azithromycin J. Biol. Chem., January 26, 2007; 282(4): 2494 - 2504. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Y. Wu, J. J. Kim, R. Reddy, W. M. Wang, D. Y. Graham, and D. H. Kwon Tetracycline-Resistant Clinical Helicobacter pylori Isolates with and without Mutations in 16S rRNA-Encoding Genes Antimicrob. Agents Chemother., February 1, 2005; 49(2): 578 - 583. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kasai, T. Kanno, Y. Endo, K. Wakasa, and Y. Tozawa Guanosine tetra- and pentaphosphate synthase activity in chloroplasts of a higher plant: association with 70S ribosomes and inhibition by tetracycline Nucleic Acids Res., October 26, 2004; 32(19): 5732 - 5741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Anokhina, A. Barta, K. H. Nierhaus, V. A. Spiridonova, and A. M. Kopylov Mapping of the second tetracycline binding site on the ribosomal small subunit of E.coli Nucleic Acids Res., May 11, 2004; 32(8): 2594 - 2597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Bauer, C. Berens, S. J. Projan, and W. Hillen Comparison of tetracycline and tigecycline binding to ribosomes mapped by dimethylsulphate and drug-directed Fe2+ cleavage of 16S rRNA J. Antimicrob. Chemother., April 1, 2004; 53(4): 592 - 599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Connell, D. M. Tracz, K. H. Nierhaus, and D. E. Taylor Ribosomal Protection Proteins and Their Mechanism of Tetracycline Resistance Antimicrob. Agents Chemother., December 1, 2003; 47(12): 3675 - 3681. [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Trieber and D. E. Taylor Mutations in the 16S rRNA Genes of Helicobacter pylori Mediate Resistance to Tetracycline J. Bacteriol., April 15, 2002; 184(8): 2131 - 2140. [Abstract] [Full Text]
|
|
|
|
 |
|
 I. Chopra and M. Roberts Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance Microbiol. Mol. Biol. Rev., June 1, 2001; 65(2): 232 - 260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Noah, M. A. Dolan, P. Babin, and P. Wollenzien Effects of Tetracycline and Spectinomycin on the Tertiary Structure of Ribosomal RNA in the Escherichia coli 30 S Ribosomal Subunit J. Biol. Chem., June 4, 1999; 274(23): 16576 - 16581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. I. Ross, E. A. Eady, J. H. Cove, and W. J. Cunliffe 16S rRNA Mutation Associated with Tetracycline Resistance in a Gram-Positive Bacterium Antimicrob. Agents Chemother., July 1, 1998; 42(7): 1702 - 1705. [Abstract] [Full Text]
|
|