Nucleic Acids Research, Vol 25, Issue 6 1233-1239, Copyright © 1997 by Oxford University Press
L Kadalayil, UM Petersen and Y Engstrom
The GATA motif is a well known positive cis -regulatory element in
vertebrates. In this work we report experimental evidence for the direct
participation of a GATA motif in the expression of the Drosophila
antibacterial peptide gene Cecropin A1 . Previously we have shown that a
kappaB-like site is necessary for Cecropin A1 gene expression. Here we
present evidence that the Drosophila Rel protein which binds to the
kappaB-like site requires an intact GATA site for maximal Dif-mediated
transactivation of the Cecropin A1 gene. We show that a Drosophila blood
cell line contains factors binding specifically to the GATA motif of the
Cecropin A1 gene. The GATA binding activity is likely to include member(s)
of the GATA family of transcriptional regulators. We show that the
promoters of several inducible insect immune genes possess GATA sites 0-12
base pairs away from kappaB-like sites in functionally important promoter
regions. Clusters of GATA and kappaB sites are also observed in the
promoters of two important mammalian immune genes, namely IL6 and IL3. The
consistent proximity of GATA and kappaB sites appears to be a common theme
in the immune gene expression of insects and mammals.
ARTICLES
Adjacent GATA and kappa B-like motifs regulate the expression of a Drosophila immune gene
Department of Molecular Biology, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-106 91, Sweden.
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