Nucleic Acids Research, Vol 25, Issue 6 1240-1247, Copyright © 1997 by Oxford University Press
T Macatee, YW Jiang, DJ Stillman and SY Roth
Sin4p is a component of a mediator complex associated with the C- terminal
domain of RNA polymerase II and SIN4 is required for proper regulation of
several genes in yeast, including the HO endonuclease gene, glucose
repressible genes and MATa cell-specific genes. Previous studies indicated
that SIN4 may influence transcription through changes in the organization
of chromatin. We have examined a specific chromatin structure associated
with MATa cell-specific repression in sin4 MATalpha cells to determine if
SIN4 is required for nucleosome positioning. Although the loss of SIN4 has
no effect on nucleosome location, we find that the sensitivity of bulk
chromatin from sin4 cells to micrococcal nuclease digestion is strikingly
increased relative to chromatin from isogenic wild-type cells. The nuclease
hypersensitivity of chromatin from sin4 cells is not related to gross
alterations in histone gene expression or to bulk increases in histone
modification. Our experiments suggest that SIN4 directly or indirectly
regulates a global aspect of chromatin accessibility, providing a molecular
basis for phenotypic similarities between sin4 mutations and mutations in
histones.
ARTICLES
Global alterations in chromatin accessibility associated with loss of SIN4 function
Department of Biochemistry and Molecular Biology, University of Texas M.D.Anderson Cancer Center, Houston, TX 77030, USA.
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