Nucleic Acids Research, Vol 25, Issue 7 1339-1346, Copyright © 1997 by Oxford University Press
AS Krasilnikov, IG Panyutin, GM Samadashwily, R Cox, YS Lazurkin and SM Mirkin
Pyrimidine/purine/purine triplexes are known to inhibit DNA polymerization.
Here we have studied the mechanisms of this inhibition by comparing the
efficiency of Vent DNA polymerase on triplex- and duplex-containing
templates at different temperatures, Mg2+concentrations and time intervals
with the thermal stability of the corresponding structures. Our results
show that triplexes can only be by-passed at temperatures where thermal
denaturation initiates, while duplexes, in contrast, are overcome at
temperatures where they are quite stable. These results show that DNA
polymerase cannot untangle triplex regions within DNA templates and seems
to entirely depend on their thermal fluctuations. The high stability of
triplexes at physiological temperatures and ambient conditions make them a
barrier to polymerization.
ARTICLES
Mechanisms of triplex-caused polymerization arrest
Department of Genetics, University of Illinois at Chicago, Chicago, IL 60607, USA.
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