Nucleic Acids Research, Vol 25, Issue 7 1458-1466, Copyright © 1997 by Oxford University Press
PP Sayeski, D Wang, K Su, IO Han and JE Kudlow
Glutamine:fructose-6-phosphate amidotransferase (GFAT) is the enzyme that
is rate limiting in the synthesis of glucosamine and hexosamines.
Glucosamine has been proposed to contribute to the glucotoxicity of
diabetes. Evidence that the gene encoding GFAT is transcriptionally
regulated prompted us to clone and characterize its promoter. The position
of the mouse GFAT promoter relative to the translational start site was
located by primer extension and found to be 149 bp upstream of the
translational start site. A 1.9 kb SacI fragment of the GFAT gene was found
to contain the promoter and 88 bp of sequence downstream of the
transcriptional start site. This promoter segment could drive expression of
a luciferase reporter gene, could confer correct transcriptional initiation
to the reporter and could confer the EGF- responsiveness previously
observed in the native gene. The mouse GFAT promoter lacks a canonical TATA
box and has several GC boxes within a highly GC-rich region. Deletional
analysis of the promoter indicated that a proximal element extending to
-120 relative to the transcriptional start site could confer reporter
expression at a level of 57% of the 1.9 kb construct. Detailed analysis of
this proximal region by DNase I footprinting, electrophoretic mobility
shift assays and site-directed mutagenesis indicated that Sp1 binds to
three elements in this proximal promoter segment and plays a vital role in
regulation of transcription from this gene.
ARTICLES
Cloning and partial characterization of the mouse glutamine:fructose-6- phosphate amidotransferase (GFAT) gene promoter
Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. R. James, A. Ingram, H. Ly, K. Thai, L. Cai, and J. W. Scholey Angiotensin II activates the GFAT promoter in mesangial cells Am J Physiol Renal Physiol, July 1, 2001; 281(1): F151 - F162. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Chang, K. Su, J. R. Baker, X. Yang, A. J. Paterson, and J. E. Kudlow Phosphorylation of Human Glutamine:Fructose-6-phosphate Amidotransferase by cAMP-dependent Protein Kinase at Serine 205 Blocks the Enzyme Activity J. Biol. Chem., July 14, 2000; 275(29): 21981 - 21987. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. P. Sayeski, M. S. Ali, S. J. Frank, and K. E. Bernstein The Angiotensin II-dependent Nuclear Translocation of Stat1 Is Mediated by the Jak2 Protein Motif 231YRFRR J. Biol. Chem., March 23, 2001; 276(13): 10556 - 10563. [Abstract] [Full Text] [PDF]
|
|