Nucleic Acids Research, Vol 25, Issue 8 1531-1536, Copyright © 1997 by Oxford University Press
K Chowdhury, P Bonaldo, M Torres, A Stoykova and P Gruss
A large scale insertional mutagenesis experiment was performed in embryonic
stem (ES) cells by introducing two types of gene trap vectors into the
genome. These cell lines carrying mutations were introduced into the mouse
germline. In order to assess the feasibility of a large scale cloning of
the targeted genes from these lines, we have isolated and characterized 55
trapped exons from the corresponding ES cells. Analysis of the data has
revealed that vectors containing or lacking an internal ribosome entry site
(IRES) can integrate into the ES cell genome stochastically. The targeted
genes comprise 30% known genes, 20% expressed sequence tags (ESTs) and 50%
novel or unknown genes. The known genes belong to several major classes and
represent complete or partial knockouts. Using currently available methods
or modifications of them, it should be feasible to do a large scale cloning
of trapped genes from the mouse ES cell lines.
ARTICLES
Evidence for the stochastic integration of gene trap vectors into the mouse germline
Department of Molecular Cell Biology, Max Planck Institute of Biophysical Chemistry, Am Fassberg, D-37077 Gottingen, Germany. kchawdh@gwdg.de
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