Nucleic Acids Research, Vol 25, Issue 9 1685-1693, Copyright © 1997 by Oxford University Press
SJ Bourgerie, CM Michan, MS Thomas, SJ Busby and EI Hyde
The Escherichia coli melR gene encodes MelR protein which is a member of
the AraC/XylS family of bacterial transcription activators. The function of
MelR was investigated by making a targeted deletion in the melR gene of the
Escherichia coli chromosome. MelR is a transcription activator essential
for melibiose- dependent expression of the melAB operon which is needed for
bacterial growth with melibiose as a carbon source. To investigate the
interactions of MelR at the melAB promoter, both full length MelR and a
shortened derivative, MelR173, containing the C-terminal DNA-binding
domain, were purified as fusions to glutathione- S -transferase. Circular
permutation studies show that both full-length MelR and MelR173 induce an
apparent bend upon binding to target sites at the melAB promoter. Bound
full-length MelR, but not MelR173, can oligomerise to form larger complexes
that are likely to be involved in transcription activation.
ARTICLES
DNA binding and DNA bending by the MelR transcription activator protein from Escherichia coli
School of Biochemistry, The University of Birmingham, PO Box 363, Birmingham B15 2TT, UK.
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