Nucleic Acids Research, Vol 25, Issue 9 1720-1726, Copyright © 1997 by Oxford University Press
R Harrod and PS Lovett
catA86 is the second gene in a constitutively transcribed, two-gene operon
cloned from Bacillus pumilus . The region that intervenes between the
upstream gene, termed the leader, and the catA86 coding sequence contains a
pair of inverted repeat sequences which cause sequestration of the catA86
ribosome binding site in mRNA secondary structure. As a consequence, the
catA86 coding sequence is untranslatable in the absence of inducer.
Translation of the catA86 coding sequence is induced by chloramphenicol in
Gram-positives and induction requires a function of the leader coding
sequence. The leader- encoded peptide has been proposed to instruct its
translating ribosome to pause at leader codon 6, enabling chloramphenicol
to stall the ribosome at that site. Ribosome stalling causes
destabilization of the RNA secondary structure, exposing the catA86
ribosome binding site, allowing activation of its translation. A comparable
mechanism of induction by chloramphenicol has been proposed for the
regulated cmlA gene from Gram-negative bacteria. The catA86 and cmlA
leader-encoded peptides are in vitro inhibitors of peptidyl transferase,
which is thought to be the basis for selection of the site of ribosome
stalling. Both leader-encoded peptides have been shown to alter the
secondary structure of Escherichia coli 23S rRNA in vitro. All
peptide-induced changes in rRNA conformation are within domains IV and V,
which contains the peptidyl transferase center. Here we demonstrate that
the leader peptides alter the conformation of domains IV and V of large
subunit rRNA from yeast and a representative of the Archaea. The rRNA
target for binding the leader peptides is therefore conserved across
kingdoms.
ARTICLES
Leader peptides of inducible chloramphenicol resistance genes from gram- positive and gram-negative bacteria bind to yeast and Archaea large subunit rRNA
Department of Biological Sciences, University of Maryland-Baltimore County, Baltimore, MD 21250, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Wang, E. R. Rotman, N. B. Shoemaker, and A. A. Salyers Translational Control of Tetracycline Resistance and Conjugation in the Bacteroides Conjugative Transposon CTnDOT J. Bacteriol., April 15, 2005; 187(8): 2673 - 2680. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Fang, C. C. Spevak, C. Wu, and M. S. Sachs A nascent polypeptide domain that can regulate translation elongation PNAS, March 23, 2004; 101(12): 4059 - 4064. [Abstract] [Full Text] [PDF]
|
|