Nucleic Acids Research, Vol 25, Issue 9 1766-1773, Copyright © 1997 by Oxford University Press
K Akagi, V Sandig, M Vooijs, M Van der Valk, M Giovannini, M Strauss and A Berns
Conditional mutant mice equipped with heterologous recombination systems
(Cre/lox or Flp/frt) are promising for studying tissue-specific gene
function and for designing better models of human diseases. The utility of
these mice depends on the cell target specificity, on the efficiency and on
the control over timing of gene (in)activation. We have explored the
utility of adenoviral vectors and transgenic mice expressing Cre under the
control of tissue-specific promoters to achieve Cre/lox-mediated somatic
recombination of the LacZ reporter gene, using a newly generated flox LacZ
mouse strain. When adeno Cre viruses were administered via different
routes, recombination and expression of LacZ was detected in a wide range
of tissues. Whereas in liverbeta-galactosidase activity was quickly lost by
turnover of expressing cells, even though the recombined allele was
retained,beta- galactosidase in other tissues persisted for many months.
Our data indicate that the flox LacZ transgenic line can be utilized
effectively to monitor the level and functionality of Cre protein produced
upon infection with adeno Cre virus or upon crossbreeding with different
Cre transgenic lines.
ARTICLES
Cre-mediated somatic site-specific recombination in mice
Division of Molecular Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.
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