Nucleic Acids Research, Vol 26, Issue 10 2255-2264, Copyright © 1998 by Oxford University Press
T Rein, ML DePamphilis and H Zorbas
Intense interest in the biological roles of DNA methylation, particularly
in eukaryotes, has produced at least eight different methods for
identifying 5-methylcytosine and related modifications in DNA genomes.
However, the utility of each method depends not only on its simplicity but
on its specificity, resolution, sensitivity and potential artifacts. Since
these parameters affect the interpretation of data, they should be
considered in any application. Therefore, we have outlined the principles
and applications of each method, quantitatively evaluated their
specificity,resolution and sensitivity, identified potential artifacts and
suggested solutions, and discussed a paradox in the distribution of m5C in
mammalian genomes that illustrates how methodological limitations can
affect interpretation of data. Hopefully, the information and analysis
provided here will guide new investigators entering this exciting field.
REVIEWS
Identifying 5-methylcytosine and related modifications in DNA genomes
National Institute of Child Health and Human Development, Building 6, Room 416, National Institutes of Health, Bethesda, MD 20892-2753, USA.
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