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Nucleic Acids Research, Vol 26, Issue 10 2415-2419, Copyright © 1998 by Oxford University Press

ARTICLES

Meiotic double-strand breaks in yeast artificial chromosomes containing human DNA

G Ira, E Svetlova and J Filipski
Laboratoire de Mutagenese. Institut J. Monod. 2, place Jussieu, Tour 43, 75251 Paris, France.

Meiotic recombination in the yeast Saccharomyces cerevisiae is initiated by double-strand breaks (DSB) in chromosomal DNA. These DSB, which can be mapped in the rad 50S mutant yeast strain, are caused by a topoisomerase II-like enzyme, the protein Spo11. Evidence suggests that this protein is located in the axial element of the meiotic chromosome which implies that the DSB are located in these chromosomes in the vicinity of the bases of the DNA loops. We have found that in the yeast artificial chromosomes carrying human DNA, at the level of resolution obtained by pulsed field gel electrophoresis (PFGE), the meiotic DSB in the diploid yeast are co-localized with the DNase I hypersensitive sites (HS) in a haploid strain of yeast. These HS are located close to sequences which, under stress, have the potential to form secondary structures containing unpaired nucleotides. Clusters of such sequences could be a hallmark of the bases of the chromatin loops.
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M. Mucha, K. Lisowska, A. Goc, and J. Filipski
Nuclease-hypersensitive Chromatin Formed by a CpG Island in Human DNA Cloned as an Artificial Chromosome in Yeast
J. Biol. Chem., January 14, 2000; 275(2): 1275 - 1278.
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