Nucleic Acids Research, Vol 26, Issue 11 2541-2553, Copyright © 1998 by Oxford University Press
T Hartshorne
U3 snoRNA is required for early pre-rRNA processing events that include
cleavage of the 5' external transcribed spacer (5'ETS) and 18S rRNA
maturation. Herein, psoralen RNA crosslinking has been used to indicate
novel in vivo interactions between the minimally-sized Trypanosoma brucei
U3 snoRNA and pre-rRNAs. Two discrete U3 crosslinks were mapped to 5'ETS
sequences, then individually isolated by hybrid selection following
digestion of pre-rRNAs. Crosslink positions within these U3- site1 and
U3-site2 complexes were resolved by RNaseH digestion and primer extension
analyses. Hinge bases of U3 contacted site1 bases U140 and U142 just 3' of
the processed primary site. This is the first experimental evidence of a U3
RNA interaction adjacent to a major 5'ETS cleavage site and supports a
critical role for U3 in its processing. Highly conserved box A bases
contacted site2 base U945, 187 nt upstream of 18S-like rRNA sequences.
Site2 sequences are not required for primary processing, thus, a U3
interaction here might have roles in subsequent downstream processing
events. These results clearly demonstrated that distinct U3 snoRNA
sequences crosslinked different regions of the 5'ETS and support a model
for U3 as a multifunctional snoRNA.
ARTICLES
Distinct regions of U3 snoRNA interact at two sites within the 5' external transcribed spacer of pre-rRNAs in Trypanosoma brucei cells
Department of Biochemistry and Molecular Biology A-10, Albany Medical College, 47 New Scotland Avenue, Albany, NY 12208, USA. toinette_hartshorne@ccgateway.amc.edu
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Barth, B. Shalem, A. Hury, I. D. Tkacz, X.-h. Liang, S. Uliel, I. Myslyuk, T. Doniger, M. Salmon-Divon, R. Unger, et al. Elucidating the Role of C/D snoRNA in rRNA Processing and Modification in Trypanosoma brucei Eukaryot. Cell, January 1, 2008; 7(1): 86 - 101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Fernandez-Miragall and E. Martinez-Salas In vivo footprint of a picornavirus internal ribosome entry site reveals differences in accessibility to specific RNA structural elements J. Gen. Virol., November 1, 2007; 88(11): 3053 - 3062. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Barth, A. Hury, X.-h. Liang, and S. Michaeli Elucidating the Role of H/ACA-like RNAs in trans-Splicing and rRNA Processing via RNA Interference Silencing of the Trypanosoma brucei CBF5 Pseudouridine Synthase J. Biol. Chem., October 14, 2005; 280(41): 34558 - 34568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. BOROVJAGIN and S. A. GERBI Xenopus U3 snoRNA docks on pre-rRNA through a novel base-pairing interaction RNA, June 1, 2004; 10(6): 942 - 953. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Colley, J. D. Beggs, D. Tollervey, and D. L. J. Lafontaine Dhr1p, a Putative DEAH-Box RNA Helicase, Is Associated with the Box C+D snoRNP U3 Mol. Cell. Biol., October 1, 2000; 20(19): 7238 - 7246. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. N. Schnare, J. C. Collings, D. F. Spencer, and M. W. Gray The 28S-18S rDNA intergenic spacer from Crithidia fasciculata: repeated sequences, length heterogeneity, putative processing sites and potential interactions between U3 small nucleolar RNA and the ribosomal RNA precursor Nucleic Acids Res., September 15, 2000; 28(18): 3452 - 3461. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Antal, A. Mougin, M. Kis, E. Boros, G. Steger, G. Jakab, F. Solymosy, and C. Branlant Molecular characterization at the RNA and gene levels of U3 snoRNA from a unicellular green alga, Chlamydomonas reinhardtii Nucleic Acids Res., August 1, 2000; 28(15): 2959 - 2968. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sharma and D. Tollervey Base Pairing between U3 Small Nucleolar RNA and the 5' End of 18S rRNA Is Required for Pre-rRNA Processing Mol. Cell. Biol., September 1, 1999; 19(9): 6012 - 6019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Dunbar, S. Wormsley, T. M. Lowe, and S. J. Baserga Fibrillarin-associated Box C/D Small Nucleolar RNAs in Trypanosoma brucei. SEQUENCE CONSERVATION AND IMPLICATIONS FOR 2'-O-RIBOSE METHYLATION OF rRNA J. Biol. Chem., May 5, 2000; 275(19): 14767 - 14776. [Abstract] [Full Text] [PDF]
|
|