Nucleic Acids Research, Vol 26, Issue 11 2606-2610, Copyright © 1998 by Oxford University Press
EG Mueller, CJ Buck, PM Palenchar, LE Barnhart and JL Paulson
All organisms modify the bases of their RNA after transcription. Relatively
little is known about the functions that these chemical alterations serve
and, with very few exceptions, even less has been established regarding the
enzymology involved. One modified base of known function is 4-thiouridine
at position 8 of certain bacterial tRNAs, which serves as a photosensor for
near-UV light. A gene involved in the conversion of uridine at position 8
into 4-thiouridine has been identified by genetic screening and its role in
4-thiouridine generation has been confirmed biochemically. This same gene,
thiI , has recently been shown to play a role in thiamin biosynthesis. The
purification and characteristics of the purified protein are also reported.
ARTICLES
Identification of a gene involved in the generation of 4-thiouridine in tRNA
Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA. emueller@udel.edu
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