Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow Print PDF (369K) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (126)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Vispe, S.
Right arrow Articles by Defais, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vispe, S.
Right arrow Articles by Defais, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, Vol 26, Issue 12 2859-2864, Copyright © 1998 by Oxford University Press

ARTICLES

Overexpression of Rad51 protein stimulates homologous recombination and increases resistance of mammalian cells to ionizing radiation

S Vispe, C Cazaux, C Lesca and M Defais
Institut de Pharmacologie et de Biologie Structurale, Centre National de la Recherche Scientifique, UPR 9062, 205 route de Narbonne, 31077 Toulouse cedex, France.

Rad51 proteins share both structural and functional homologies with the bacterial recombinase RecA. The human Rad51 (HsRad51) is able to catalyse strand exchange between homologous DNA molecules in vitro . However the biological functions of Rad51 in mammals are largely unknown. In order to address this question, we have cloned hamster Rad51 cDNA and overexpressed the corresponding protein in CHO cells. We found that 2-3-fold overexpression of the protein stimulated the homologous recombination between integrated genes by 20-fold indicating that Rad51 is a functional and key enzyme of an intrachromosomal recombination pathway. Cells overexpressing Rad51 were resistant to ionizing radiation when irradiated in late S/G2phase of the cell cycle. This suggests that Rad51 participate in the repair of double-strand breaks most likely by homologous recombination involving sister chromatids formed after the S phase.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
D. Schild and C. Wiese
Overexpression of RAD51 suppresses recombination defects: a possible mechanism to reverse genomic instability
Nucleic Acids Res., November 26, 2009; (2009) gkp1063v1.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
T. Yan, Y. Seo, and T. J. Kinsella
Differential Cellular Responses to Prolonged LDR-IR in MLH1-Proficient and MLH1-Deficient Colorectal Cancer HCT116 Cells
Clin. Cancer Res., November 15, 2009; 15(22): 6912 - 6920.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
X. Liu, E. K. Han, M. Anderson, Y. Shi, D. Semizarov, G. Wang, T. McGonigal, L. Roberts, L. Lasko, J. Palma, et al.
Acquired Resistance to Combination Treatment with Temozolomide and ABT-888 Is Mediated by Both Base Excision Repair and Homologous Recombination DNA Repair Pathways
Mol. Cancer Res., October 1, 2009; 7(10): 1686 - 1692.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
J.-C. Ko, S.-C. Ciou, J.-Y. Jhan, C.-M. Cheng, Y.-J. Su, S.-M. Chuang, S.-T. Lin, C.-C. Chang, and Y.-W. Lin
Roles of MKK1/2-ERK1/2 and Phosphoinositide 3-Kinase-AKT Signaling Pathways in Erlotinib-Induced Rad51 Suppression and Cytotoxicity in Human Non-Small Cell Lung Cancer Cells
Mol. Cancer Res., August 1, 2009; 7(8): 1378 - 1389.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
J.-C. Ko, J.-H. Hong, L.-H. Wang, C.-M. Cheng, S.-C. Ciou, S.-T. Lin, M.-Y. Jheng, and Y.-W. Lin
Role of repair protein Rad51 in regulating the response to gefitinib in human non-small cell lung cancer cells
Mol. Cancer Ther., November 1, 2008; 7(11): 3632 - 3641.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
J.-C. Ko, S.-C. Ciou, C.-M. Cheng, L.-H. Wang, J.-H. Hong, M.-Y. Jheng, S.-T. Ling, and Y.-W. Lin
Involvement of Rad51 in cytotoxicity induced by epidermal growth factor receptor inhibitor (gefitinib, IressaR) and chemotherapeutic agents in human lung cancer cells
Carcinogenesis, July 1, 2008; 29(7): 1448 - 1458.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
J. Nomme, Y. Takizawa, S. F. Martinez, A. Renodon-Corniere, F. Fleury, P. Weigel, K.-i. Yamamoto, H. Kurumizaka, and M. Takahashi
Inhibition of filament formation of human Rad51 protein by a small peptide derived from the BRC-motif of the BRCA2 protein.
Genes Cells, May 1, 2008; 13(5): 471 - 481.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
V. Tripathi, S. Kaur, and S. Sengupta
Phosphorylation-dependent interactions of BLM and 53BP1 are required for their anti-recombinogenic roles during homologous recombination
Carcinogenesis, January 1, 2008; 29(1): 52 - 61.
[Abstract] [Full Text] [PDF]

Home page
 Neuro Oncol DukeHome page
S. C. Short, C. Martindale, S. Bourne, G. Brand, M. Woodcock, and P. Johnston
DNA repair after irradiation in glioma cells and normal human astrocytes
Neuro-oncol, October 1, 2007; 9(4): 404 - 411.
[Abstract] [Full Text] [PDF]

Home page
 Ann OncolHome page
M. Voso, E Fabiani, F D'Alo', F Guidi, A Di Ruscio, S Sica, L Pagano, M Greco, S Hohaus, and G Leone
Increased risk of acute myeloid leukaemia due to polymorphisms in detoxification and DNA repair enzymes
Ann. Onc., September 1, 2007; 18(9): 1523 - 1528.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
J. A.F. Hannay, J. Liu, Q.-S. Zhu, S. V. Bolshakov, L. Li, P. W.T. Pisters, A. J.F. Lazar, D. Yu, R. E. Pollock, and D. Lev
Rad51 overexpression contributes to chemoresistance in human soft tissue sarcoma cells: a role for p53/activator protein 2 transcriptional regulation
Mol. Cancer Ther., May 1, 2007; 6(5): 1650 - 1660.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
S. So, Y. Nomura, N. Adachi, Y. Kobayashi, T. Hori, Y. Kurihara, and H. Koyama
Enhanced gene targeting efficiency by siRNA that silences the expression of the Bloom syndrome gene in human cells
Genes Cells, April 1, 2006; 11(4): 363 - 371.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
G. J. Duigou and C. S. H. Young
Replication-Competent Adenovirus Formation in 293 Cells: the Recombination-Based Rate Is Influenced by Structure and Location of the Transgene Cassette and Not Increased by Overproduction of HsRad51, Rad51-Interacting, or E2F Family Proteins
J. Virol., May 1, 2005; 79(9): 5437 - 5444.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. Fan, T. S. Kumaravel, F. Jalali, P. Marrano, J. A. Squire, and R. G. Bristow
Defective DNA Strand Break Repair after DNA Damage in Prostate Cancer Cells: Implications for Genetic Instability and Prostate Cancer Progression
Cancer Res., December 1, 2004; 64(23): 8526 - 8533.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. Saleh-Gohari and T. Helleday
Conservative homologous recombination preferentially repairs DNA double-strand breaks in the S phase of the cell cycle in human cells
Nucleic Acids Res., July 13, 2004; 32(12): 3683 - 3688.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. S. Russell, K. Brady, W. E. Burgan, M. A. Cerra, K. A. Oswald, K. Camphausen, and P. J. Tofilon
Gleevec-Mediated Inhibition of Rad51 Expression and Enhancement of Tumor Cell Radiosensitivity
Cancer Res., November 1, 2003; 63(21): 7377 - 7383.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Ivanov, M. S. Cragg, J. Erenpreisa, D. Emzinsh, H. Lukman, and T. M. Illidge
Endopolyploid cells produced after severe genotoxic damage have the potential to repair DNA double strand breaks
J. Cell Sci., October 15, 2003; 116(20): 4095 - 4106.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. L. Donahue, R. Lundberg, R. Saplis, and C. Campbell
Deficient Regulation of DNA Double-strand Break Repair in Fanconi Anemia Fibroblasts
J. Biol. Chem., August 8, 2003; 278(32): 29487 - 29495.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. P. Linke, S. Sengupta, N. Khabie, B. A. Jeffries, S. Buchhop, S. Miska, W. Henning, R. Pedeux, X. W. Wang, L. J. Hofseth, et al.
p53 Interacts with hRAD51 and hRAD54, and Directly Modulates Homologous Recombination
Cancer Res., May 15, 2003; 63(10): 2596 - 2605.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
K.-i. Yoshioka, Y. Yumoto-Yoshioka, F. Fleury, and M. Takahashi
pH- and Salt-Dependent Self-Assembly of Human Rad51 Protein Analyzed as Fluorescence Resonance Energy Transfer between Labeled Proteins
J. Biochem., May 1, 2003; 133(5): 593 - 597.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. Aloyz, Z.-Y. Xu, V. Bello, J. Bergeron, F.-Y. Han, Y. Yan, A. Malapetsa, M. A. Alaoui-Jamali, A. M. V. Duncan, and L. Panasci
Regulation of Cisplatin Resistance and Homologous Recombinational Repair by the TFIIH Subunit XPD
Cancer Res., October 1, 2002; 62(19): 5457 - 5462.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. M. Kim, K. S. Paffett, J. A. Solinger, W.-D. Heyer, and J. A. Nickoloff
Spontaneous and double-strand break-induced recombination, and gene conversion tract lengths, are differentially affected by overexpression of wild-type or ATPase-defective yeast Rad54
Nucleic Acids Res., July 1, 2002; 30(13): 2727 - 2735.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Slupianek, G. Hoser, I. Majsterek, A. Bronisz, M. Malecki, J. Blasiak, R. Fishel, and T. Skorski
Fusion Tyrosine Kinases Induce Drug Resistance by Stimulation of Homology-Dependent Recombination Repair, Prolongation of G2/M Phase, and Protection from Apoptosis
Mol. Cell. Biol., June 15, 2002; 22(12): 4189 - 4201.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. Wiese, A. J. Pierce, S. S. Gauny, M. Jasin, and A. Kronenberg
Gene Conversion Is Strongly Induced in Human Cells by Double-strand Breaks and Is Modulated by the Expression of BCL-xL
Cancer Res., March 1, 2002; 62(5): 1279 - 1283.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
E. Raderschall, K. Stout, S. Freier, V. Suckow, S. Schweiger, and T. Haaf
Elevated Levels of Rad51 Recombination Protein in Tumor Cells
Cancer Res., January 1, 2002; 62(1): 219 - 225.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. Raderschall, A. Bazarov, J. Cao, R. Lurz, A. Smith, W. Mann, H.-H. Ropers, J. M. Sedivy, E. I. Golub, E. Fritz, et al.
Formation of higher-order nuclear Rad51 structures is functionally linked to p21 expression and protection from DNA damage-induced apoptosis
J. Cell Sci., January 1, 2002; 115(1): 153 - 164.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. M. Kim, C. Allen, B. M. Wagener, Z. Shen, and J. A. Nickoloff
Overexpression of human RAD51 and RAD52 reduces double-strand break-induced homologous recombination in mammalian cells
Nucleic Acids Res., November 1, 2001; 29(21): 4352 - 4360.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
K. Bishay, K. Ory, M.-F. Olivier, J. Lebeau, C. Levalois, and S. Chevillard
DNA damage-related RNA expression to assess individual sensitivity to ionizing radiation
Carcinogenesis, August 1, 2001; 22(8): 1179 - 1183.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. M. Vasquez, K. Marburger, Z. Intody, and J. H. Wilson
Manipulating the mammalian genome by homologous recombination
PNAS, July 17, 2001; 98(15): 8403 - 8410.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. J. Collis, A. Tighe, S. D. Scott, S. A. Roberts, J. H. Hendry, and G. P. Margison
Ribozyme minigene-mediated RAD51 down-regulation increases radiosensitivity of human prostate cancer cells
Nucleic Acids Res., April 1, 2001; 29(7): 1534 - 1538.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Arnaudeau, L. Rozier, C. Cazaux, M. Defais, D. Jenssen, and T. Helleday
RAD51 supports spontaneous non-homologous recombination in mammalian cells, but not the corresponding process induced by topoisomerase inhibitors
Nucleic Acids Res., February 1, 2001; 29(3): 662 - 667.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. M. N. Akkari, R. L. Bateman, C. A. Reifsteck, S. B. Olson, and M. Grompe
DNA Replication Is Required To Elicit Cellular Responses to Psoralen-Induced DNA Interstrand Cross-Links
Mol. Cell. Biol., November 1, 2000; 20(21): 8283 - 8289.
[Abstract] [Full Text]

Home page
 GeneticsHome page
D. D. G. Gessler and S. Xu
Meiosis and the Evolution of Recombination at Low Mutation Rates
Genetics, September 1, 2000; 156(1): 449 - 456.
[Abstract] [Full Text]

Home page
 GeneticsHome page
T. C. Marsh, E. S. Cole, K. R. Stuart, C. Campbell, and D. P. Romero
RAD51 Is Required for Propagation of the Germinal Nucleus in Tetrahymena thermophila
Genetics, April 1, 2000; 154(4): 1587 - 1596.
[Abstract] [Full Text]

Home page
 Nucleic Acids ResHome page
W. Li, B. Hesabi, A. Babbo, C. Pacione, J. Liu, D. J. Chen, J. A. Nickoloff, and Z. Shen
Regulation of double-strand break-induced mammalian homologous recombination by UBL1, a RAD51-interacting protein
Nucleic Acids Res., March 1, 2000; 28(5): 1145 - 1153.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
T. Haaf, E. Raderschall, G. Reddy, D. C. Ward, C. M. Radding, and E. I. Golub
Sequestration of Mammalian Rad51-Recombination Protein into Micronuclei
J. Cell Biol., January 11, 1999; 144(1): 11 - 20.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X.-D. Yao and P. Elias
Recombination during Early Herpes Simplex Virus Type 1 Infection Is Mediated by Cellular Proteins
J. Biol. Chem., January 19, 2001; 276(4): 2905 - 2913.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Reiss, I. Schubert, K. Kopchen, E. Wendeler, J. Schell, and H. Puchta
RecA stimulates sister chromatid exchange and the fidelity of double-strand break repair, but not gene targeting, in plants transformed by Agrobacterium
PNAS, March 28, 2000; 97(7): 3358 - 3363.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Xia, D. G. Taghian, J. S. DeFrank, Z.-C. Zeng, H. Willers, G. Iliakis, and S. N. Powell
Deficiency of human BRCA2 leads to impaired homologous recombination but maintains normal nonhomologous end joining
PNAS, July 17, 2001; 98(15): 8644 - 8649.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.