Nucleic Acids Research, Vol 26, Issue 12 2989-2994, Copyright © 1998 by Oxford University Press
SJ Park, ES Yang, J Kim-Ha and YJ Kim
Rbp9 is an RNA binding protein expressed mainly in the central nervous
system of adult Drosophilamelanogaster. Rbp9 shares a high degree of
sequence similarity with human neural proteins referred to as Hu antigens.
Hu antigens bind to U-rich mRNA destabilizing elements with a high affinity
and, thus, have been implicated as regulators of mRNA stability. Using in
vitro RNA binding assays, we found that Rbp9 binds strongly to poly U
sequences. We then employed a Selex system to identify a consensus Rbp9
binding site (UUUXUUUU). Information obtained from the Selex results
allowed the detection of two repeats of the Rbp9 consensus binding sequence
in the 3' untranslated region of extramacrochaetae mRNA. UV crosslinking
experiments demonstrated that Rbp9 interactsspecifically with emc mRNA. The
requirement of Rbp9 protein in the down regulation of emc mRNA was
confirmed by northern (RNA) analysis, which revealed that the level of emc
mRNA increased 10- fold in rbp9 mutant flies. Taken together with the in
vitro RNA binding results, the genetic evidence obtained strongly supports
the hypothesis that Rbp9 functions as a regulator of RNA stability.
ARTICLES
Down regulation of extramacrochaetae mRNA by a Drosophila neural RNA binding protein Rbp9 which is homologous to human Hu proteins
Laboratory of Molecular Genetics, Center for Basic Research, Samsung Biomedical Research Institute, Ilwon-dong 50, Kangnam-ku, Seoul 135- 230, Korea.
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