Nucleic Acids Research, Vol 26, Issue 13 3077-3083, Copyright © 1998 by Oxford University Press
J Qiu, MX Guan, AM Bailis and B Shen
Two closely related genes, EXO1 and DIN 7, in the budding yeast
Saccharomyces cerevisiae have been found to be sequence homologs of the
exo1 gene from the fission yeast Schizosaccharomyces pombe . The proteins
encoded by these genes belong to the Rad2/XPG and Rad27/FEN-1 families,
which are structure-specific nucleases functioning in DNA repair. An XPG
nuclease deficiency in humans is one cause of xeroderma pigmentosum and
those afflicted display a hypersensitivity to UV light. Deletion of the
RAD2 gene in S. cerevisiae also causes UV hypersensitivity, due to a defect
in nucleotide excision repair (NER), but residual UV resistance remains. In
this report, we describe evidence for the residual repair of UV damage to
DNA that is dependent upon Exo1 nuclease. Expression of the EXO1 gene is UV
inducible. Genetic analysis indicates that the EXO1 gene is involved in a
NER- independent pathway for UV repair, as exo1 rad2 double mutants are
more sensitive to UV than either the rad2 or exo1 single mutants. Since the
roles of EXO1 in mismatch repair and recombination have been established,
double mutants were constructed to examine the possible relationship
between the role of EXO1 in UV resistance and its roles in other pathways
for repair of UV damaged DNA. The exo1 msh2 , exo1 rad51 , rad2 rad51 and
rad2 msh2 double mutants were all more sensitive to UV than their
respective pairs of single mutants. This suggests that the observed UV
sensitivity of the exo1 deletion mutant is unlikely to be due to its
functional deficiencies in MMR, recombination or NER. Further, it suggests
that the EXO1 , RAD51 and MSH2 genes control independent mechanisms for the
maintenance of UV resistance.
ARTICLES
Saccharomyces cerevisiae exonuclease-1 plays a role in UV resistance that is distinct from nucleotide excision repair
Department of Cell and Tumor Biology, The Beckman Research Institute of City of Hope, Duarte, CA 91010-3000, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Z. Guo, L. Qian, R. Liu, H. Dai, M. Zhou, L. Zheng, and B. Shen Nucleolar Localization and Dynamic Roles of Flap Endonuclease 1 in Ribosomal DNA Replication and Damage Repair Mol. Cell. Biol., July 1, 2008; 28(13): 4310 - 4319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Yan, X. Li, G. Faye, and M.-X. Guan Mutations in MTO2 Related to tRNA Modification Impair Mitochondrial Gene Expression and Protein Synthesis in the Presence of a Paromomycin Resistance Mutation in Mitochondrial 15 S rRNA J. Biol. Chem., August 12, 2005; 280(32): 29151 - 29157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Nakada, Y. Hirano, and K. Sugimoto Requirement of the Mre11 Complex and Exonuclease 1 for Activation of the Mec1 Signaling Pathway Mol. Cell. Biol., November 15, 2004; 24(22): 10016 - 10025. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Wei, A. B. Clark, E. Wong, M. F. Kane, D. J. Mazur, T. Parris, N. K. Kolas, R. Russell, H. Hou Jr., B. Kneitz, et al. Inactivation of Exonuclease 1 in mice results in DNA mismatch repair defects, increased cancer susceptibility, and male and female sterility Genes & Dev., March 1, 2003; 17(5): 603 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. T. Tran, J. A. Simon, and R. M. Liskay Interactions of Exo1p with components of MutLalpha in Saccharomyces cerevisiae PNAS, July 24, 2001; (2001) 161175998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Negritto, J. Qiu, D. O. Ratay, B. Shen, and A. M. Bailis Novel Function of Rad27 (FEN-1) in Restricting Short-Sequence Recombination Mol. Cell. Biol., April 1, 2001; 21(7): 2349 - 2358. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. M. Kirchner, H. Tran, and M. A. Resnick A DNA Polymerase {epsilon} Mutant That Specifically Causes +1 Frameshift Mutations Within Homonucleotide Runs in Yeast Genetics, August 1, 2000; 155(4): 1623 - 1632. [Abstract] [Full Text]
|
|
|
|
|
|
T. Sokolsky and E. Alani EXO1 and MSH6 Are High-Copy Suppressors of Conditional Mutations in the MSH2 Mismatch Repair Gene of Saccharomyces cerevisiae Genetics, June 1, 2000; 155(2): 589 - 599. [Abstract] [Full Text]
|
|
|
|
|
|
B.-I. Lee and D. M. Wilson III The RAD2 Domain of Human Exonuclease 1 Exhibits 5' to 3' Exonuclease and Flap Structure-specific Endonuclease Activities J. Biol. Chem., December 31, 1999; 274(53): 37763 - 37769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Qiu, Y. Qian, V. Chen, M.-X. Guan, and B. Shen Human Exonuclease 1 Functionally Complements Its Yeast Homologues in DNA Recombination, RNA Primer Removal, and Mutation Avoidance J. Biol. Chem., June 18, 1999; 274(25): 17893 - 17900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. T. Tran, J. A. Simon, and R. M. Liskay Interactions of Exo1p with components of MutLalpha in Saccharomyces cerevisiae PNAS, August 14, 2001; 98(17): 9760 - 9765. [Abstract] [Full Text] [PDF]
|
|