Nucleic Acids Research, Vol 26, Issue 13 3097-3103, Copyright © 1998 by Oxford University Press
FEM Paulin, SA Chappell and AE Willis
A 340 nucleotide section of the c- myc 5' untranslated region (UTR)
contains an internal ribosome entry segment. We have described previously a
mutation in this region of RNA in cell lines derived from patients with
multiple myeloma (MM) which exhibit increased expression of c- myc protein
by an aberrant translational mechanism. In this study we show by
electrophoretic mobility shift assays (EMSA), north-western blotting and UV
cross-linking that radiolabelled c- myc 5' UTR RNA transcripts which
harbour the mutation cause enhanced binding of cellular proteins. In
addition, we also demonstrate that an MM derived cell line possesses an
altered repertoire of RNA binding proteins. Our data suggest that the
deregulated expression of c -myc in MM could result both from the effect of
the mutation and the additional proteins which are present in these cell
types.
ARTICLES
A single nucleotide change in the c-myc internal ribosome entry segment leads to enhanced binding of a group of protein factors
Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, UK.
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