Nucleic Acids Research, Vol 26, Issue 13 3154-3158, Copyright © 1998 by Oxford University Press
SH Leem, CN Chung, Y Sunwoo and H Araki
The transcript levels of DNA replication genes and some recombination genes
in Saccharomyces cerevisiae fluctuate and peak at the G1/S boundary in the
mitotic cell cycle. This fluctuation is regulated by MCB (Mlu I cell cycle
box) elements which are bound by the DSC1/MBF1 complex consisting of Swi6
and Mbp1. It is also known that some of the MCB-regulated genes are induced
by treatment with DNA damaging agents and in meiosis. In this report, the
function of SWI6 in meiosis was investigated. Delta swi6 cells underwent
sporulation as did wild-type cells. However, the deletion mutant cells
showed reduced spore viability and lower frequency of recombination. The
transcript levels of the recombination genes RAD51 and RAD54 , which have
MCB elements, were reduced in Delta swi6 cells. The transcript levels of
SWI6 itself were also induced and declined in meiosis. Furthermore, an
increased dosage of SWI6 enhanced the transcript level of the RAD51 gene
and also the recombination frequency in meiosis. These results suggest that
SWI6 enhances the expression level of the recombination genes in meiosis in
a dosage-dependent manner, which results in an effect on the frequency of
meiotic recombination.
ARTICLES
Meiotic role of SWI6 in Saccharomyces cerevisiae
Department of Biochemistry and Molecular Biology, Research Institute for Microbial Diseases, Osaka University, Yamada-oka 3-1, Suita, Osaka 565-0871, Japan.
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