Nucleic Acids Research, Vol 26, Issue 13 3202-3207, Copyright © 1998 by Oxford University Press
NM Fast, AJ Roger, CA Richardson and WF Doolittle
The removal of introns from pre-messenger RNA is mediated by the
spliceosome, a large complex composed of many proteins and five small
nuclear RNAs (snRNAs). Of the snRNAs, the U6 and U2 snRNAs are the most
conserved in sequence, as they interact extensively with each other and
also with the intron, in several base pairings that are necessary for
splicing. We have isolated and sequenced the genes encoding both U6 and U2
snRNAs from the intracellularly parasitic microsporidian Nosema locustae .
Both genes are expressed. Both RNAs can be folded into secondary structures
typical of other known U6 and U2 snRNAs. In addition, the N.locustae U6 and
U2 snRNAs have the potential to base pair in the functional intermolecular
interactions that have been characterized by extensive analyses in yeast
and mammalian systems. These results indicate that the N.locustae U6 and U2
snRNAs may be functional components of an active spliceosome, even though
introns have not yet been found in microsporidian genes.
ARTICLES
U2 and U6 snRNA genes in the microsporidian Nosema locustae: evidence for a functional spliceosome
Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada. nfast@is2.dal.ca
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