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Nucleic Acids Research, Vol 26, Issue 14 3348-3349, Copyright © 1998 by Oxford University Press

ARTICLES

BfiI, a restriction endonuclease from Bacillus firmus S8120, which recognizes the novel non-palindromic sequence 5'-ACTGGG(N)5/4-3'

J Vitkute, Z Maneliene, M Petrusyte and A Janulaitis
Institute of Biotechnology, Graiciuno 8, 2028 Vilnius, Lithuania.

A new type IIS restriction endonuclease Bfi I hasbeen partially purified from Bacillus firmus S8120. Bfi I recognizes the non- palindromic hexanucleotide sequence 5'-ACTGGG(N)5/4-3' and makes a staggered cut at the fifth base pair downstream of the recognition sequence on the upper strand, producing a single base 3' protruding end.
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