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Nucleic Acids Research, Vol 26, Issue 14 3447-3448, Copyright © 1998 by Oxford University Press

ARTICLES

Efficient modification of a human chromosome by telomere-directed truncation in high homologous recombination-proficient chicken DT40 cells

Y Kuroiwa, T Shinohara, T Notsu, K Tomizuka, H Yoshida, S Takeda, M Oshimura and I Ishida
Central Laboratories for Key Technology, Kirin Brewery Company Ltd, 1- 13-5 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan.

Truncation of human chromosomes at desired sites by homologous recombination techniques enables functional and structural analyses of human chromosomes and development of human artificial chromosomes. However, this targeted truncation has been inefficient. We describe here an efficient method for targeted truncation in the chicken DT40 cells with a high homologous recombination rate. The human chromosome 22 was transferred into DT40 cells, where human telomeric repeat (TTAGGG)n was targeted to the LIF locus on the chromosome. Molecular and cytogenetic analyses showed that the predicted truncation at the LIF locus occurred in all of the targeted clones.
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